DNA nanostructure-based ultrasensitive electrochemical microRNA biosensor

CAS OpenIR > 中科院上海应用物理研究所2011-2019年

	DNA nanostructure-based ultrasensitive electrochemical microRNA biosensor
	Wen, Yanli; Liu, Gang; Pei, Hao（裴浩）; Li, Lanying; Xu, Qin; Liang, Wen; Li, Yan; Xu, Li; Ren, Suzhen; Fan, Chunhai（樊春海）
	2013
Source Publication	METHODS
ISSN	1046-2023; 1095-9130
Volume	64 Issue:3 Pages:CONCATENATE(Sheet1!I91,-Sheet1!J91)
Abstract	MicroRNAs (miRNAs) are key regulators of a wide range of cellular processes, and have been identified as promising cancer biomarkers due to their stable presence in serum. As an surface-based electrochemical biosensors which offer great opportunities for low-cost, point-of-care tests (POCTs) of disease-associated miRNAs. Nevertheless, the sensitivity of miRNA sensors is often limited by mass transport and the surface crowding effect at the water-electrode interface. Here, we present a protocol as well as guidelines for ultrasensitive detection of miRNA with DNA nanostructure-based electrochemical miRNA biosensor. By employing the three-dimensional DNA nanostructure-based interfacial engineering approach, we can directly detect as few as attomolar (<1000 copies) miRNAs with high single-base discrimination ability. Since this ultrasensitive electrochemical miRNA sensor (EMRS) is highly reproducible and essentially free of prior target labeling and PCR amplification, it can conveniently and reliably analyze miRNA expression levels in clinical samples from esophageal squamous cell carcinoma (ESCC) patients. (c) 2013 Elsevier Inc. All rights reserved.
Indexed By	SCI
Language	英语
Funding Project	应物所项目组
WOS ID	WOS:000328309600014
Citation statistics	Cited Times:51[WOS] [WOS Record] [Related Records in WOS]
Document Type	期刊论文
Identifier	http://ir.sinap.ac.cn/handle/331007/13720
Collection	中科院上海应用物理研究所2011-2019年
Recommended Citation GB/T 7714	Wen, Yanli,Liu, Gang,Pei, Hao,et al. DNA nanostructure-based ultrasensitive electrochemical microRNA biosensor[J]. METHODS,2013,64(3):CONCATENATE(Sheet1!I91,-Sheet1!J91).
APA	Wen, Yanli.,Liu, Gang.,Pei, Hao.,Li, Lanying.,Xu, Qin.,...&Fan, Chunhai.(2013).DNA nanostructure-based ultrasensitive electrochemical microRNA biosensor.METHODS,64(3),CONCATENATE(Sheet1!I91,-Sheet1!J91).
MLA	Wen, Yanli,et al."DNA nanostructure-based ultrasensitive electrochemical microRNA biosensor".METHODS 64.3(2013):CONCATENATE(Sheet1!I91,-Sheet1!J91).

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