CAS OpenIR  > 中科院上海应用物理研究所2011-2019年
Graphene-based nanoprobes and a prototype optical biosensing platform
Xu, Hui; Wang, Dongfang; He, Shijiang; Li, Jiang; Feng, Bianying; Ma, Peijun; Xu, Pingping; Gao, Shanmin; Zhang, Shengxiao; Liu, Quanwen; Lu, Jianxin; Song, Shiping(宋世平); Fan, Chunhai(樊春海)
AbstractBiochemical and biomedical applications of graphene are critically dependent on the interaction between biomolecules and the nanomaterial. In this work, we developed a graphene-based signal-amplification nanoprobe by combining anti-immunoglobulin G (anti-IgG) and horseradish peroxidase (HRP) with graphene oxide (GO). The structure and function of HRP in the nano-interface of GO were firstly investigated, which demonstrated that the enzyme retained 78% of its native activity and 77% of its native a-helix content HRP and anti-IgG were then co-adsorbed onto GO to form bifunctional nanoprobes. The nanoprobes provide both improved binding ability and signal-amplification ability. Comparing with conventional bioconjugates such as enzyme-linked antibody, co-adsorption could avoid chemical conjugation between biomolecules, keeping their bioactivity well. As an example for their application, the nanoprobes were used to obtain amplified signals in a sandwich-type immunoassay for cancer marker, instead of conventional colorimetric conjugates. This approach provided a detection limit of 10 pg/mL alpha-fetoprotein (AFP), which was much more sensitive than conventional enzyme-linked immunosorbent assay (ELISA) methods. The easily fabricated GO-based nanoprobes have the potential to become universal probes for molecular diagnostics. (C) 2013 Elsevier B.V. All rights reserved.
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Funding Project应物所项目组
WOS IDWOS:000325188200040
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Cited Times:28[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
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GB/T 7714
Xu, Hui,Wang, Dongfang,He, Shijiang,et al. Graphene-based nanoprobes and a prototype optical biosensing platform[J]. BIOSENSORS & BIOELECTRONICS,2013,50:CONCATENATE(Sheet1!I141,-Sheet1!J141).
APA Xu, Hui.,Wang, Dongfang.,He, Shijiang.,Li, Jiang.,Feng, Bianying.,...&Fan, Chunhai.(2013).Graphene-based nanoprobes and a prototype optical biosensing platform.BIOSENSORS & BIOELECTRONICS,50,CONCATENATE(Sheet1!I141,-Sheet1!J141).
MLA Xu, Hui,et al."Graphene-based nanoprobes and a prototype optical biosensing platform".BIOSENSORS & BIOELECTRONICS 50(2013):CONCATENATE(Sheet1!I141,-Sheet1!J141).
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