CAS OpenIR  > 中科院上海应用物理研究所2011-2019年
Highly sensitive recognition of Pb2+ using Pb2+ triggered exonuclease aided DNA recycling
Xu, Hui; Xu, Pingping; Gao, Shanmin; Zhang, Shengxiao; Zhao, Xingchun; Fan, Chunhai(樊春海); Zuo, Xiaolei(左小磊)
2013
Source PublicationBIOSENSORS & BIOELECTRONICS
ISSN0956-5663
Volume47Pages:CONCATENATE(Sheet1!I151,-Sheet1!J151)
AbstractHere, we have demonstrated an ultra-high sensitive detection platform with the detection limit of 5 pM for an environmental toxin-Pb2+. We designed a Pb2+ triggered exonuclease aided DNA recycling system to improve the detection sensitivity. In our system, a Pb2+ dependent 8-17 DNAzyme and its substrate were used to form hybridization duplex. In the presence of Pb2+, the substrate was cleaved and disassociated from the duplex. Then, the released 8-17 DNAzyme was used as a target of the exonuclease aided DNA recycling system which can amplify the fluorescence signal by recycling the 8-17 DNAzyme continuously. Then, the sensitive Pb2+ detection are accomplished and the detection limit of Pb2+ was down to 5 pM which is about 1000 times lower than the traditional detection method based on the 8-17 DNAzyme. (C) 2013 Elsevier B.V. All rights reserved.
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000320481900079
Citation statistics
Cited Times:26[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/13768
Collection中科院上海应用物理研究所2011-2019年
Recommended Citation
GB/T 7714
Xu, Hui,Xu, Pingping,Gao, Shanmin,et al. Highly sensitive recognition of Pb2+ using Pb2+ triggered exonuclease aided DNA recycling[J]. BIOSENSORS & BIOELECTRONICS,2013,47:CONCATENATE(Sheet1!I151,-Sheet1!J151).
APA Xu, Hui.,Xu, Pingping.,Gao, Shanmin.,Zhang, Shengxiao.,Zhao, Xingchun.,...&Zuo, Xiaolei.(2013).Highly sensitive recognition of Pb2+ using Pb2+ triggered exonuclease aided DNA recycling.BIOSENSORS & BIOELECTRONICS,47,CONCATENATE(Sheet1!I151,-Sheet1!J151).
MLA Xu, Hui,et al."Highly sensitive recognition of Pb2+ using Pb2+ triggered exonuclease aided DNA recycling".BIOSENSORS & BIOELECTRONICS 47(2013):CONCATENATE(Sheet1!I151,-Sheet1!J151).
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