CAS OpenIR  > 中科院上海应用物理研究所2011-2020年
Imaging cells and sub-cellular structures with ultrahigh resolution full-field X-ray microscopy
Chien, C. C.; Tseng, P. Y.; Chen, H. H.; Hua, T. E.; Chen, S. T.; Chen, Y. Y.; Leng, W. H.; Wang, C. H.; Hwu, Y.; Yin, G. C.; Liang, K. S.; Chen, F. R.; Chu, Y. S.; Yeh, H. I.; Yang, Y. C.; Yang, C. S.; Zhang, G. L.; Je, J. H.; Margaritondo, G.
2013
Source PublicationBIOTECHNOLOGY ADVANCES
ISSN0734-9750
Volume31Issue:3Pages:CONCATENATE(Sheet1!I159,-Sheet1!J159)
AbstractOur experimental results demonstrate that full-field hard-X-ray microscopy is finally able to investigate the internal structure of cells in tissues. This result was made possible by three main factors: the use of a coherent (synchrotron) source of X-rays, the exploitation of contrast mechanisms based on the real part of the refractive index and the magnification provided by high-resolution Fresnel zone-plate objectives. We specifically obtained high-quality microradiographs of human and mouse cells with 29 nm Rayleigh spatial resolution and verified that tomographic reconstruction could be implemented with a final resolution level suitable for subcellular features. We also demonstrated that a phase retrieval method based on a wave propagation algorithm could yield good subcellular images starting from a series of defocused microradiographs. The concluding discussion compares cellular and subcellular hard-X-ray microradiology with other techniques and evaluates its potential impact on biomedical research. (C) 2012 Elsevier Inc. All rights reserved.
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000317441900004
Citation statistics
Cited Times:14[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/13775
Collection中科院上海应用物理研究所2011-2020年
Recommended Citation
GB/T 7714
Chien, C. C.,Tseng, P. Y.,Chen, H. H.,et al. Imaging cells and sub-cellular structures with ultrahigh resolution full-field X-ray microscopy[J]. BIOTECHNOLOGY ADVANCES,2013,31(3):CONCATENATE(Sheet1!I159,-Sheet1!J159).
APA Chien, C. C..,Tseng, P. Y..,Chen, H. H..,Hua, T. E..,Chen, S. T..,...&Margaritondo, G..(2013).Imaging cells and sub-cellular structures with ultrahigh resolution full-field X-ray microscopy.BIOTECHNOLOGY ADVANCES,31(3),CONCATENATE(Sheet1!I159,-Sheet1!J159).
MLA Chien, C. C.,et al."Imaging cells and sub-cellular structures with ultrahigh resolution full-field X-ray microscopy".BIOTECHNOLOGY ADVANCES 31.3(2013):CONCATENATE(Sheet1!I159,-Sheet1!J159).
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