Knowledge Management System Of Shanghai Institute of Applied Physics, CAS
| Imaging cells and sub-cellular structures with ultrahigh resolution full-field X-ray microscopy | |
| Chien, C. C.; Tseng, P. Y.; Chen, H. H.; Hua, T. E.; Chen, S. T.; Chen, Y. Y.; Leng, W. H.; Wang, C. H.; Hwu, Y.; Yin, G. C.; Liang, K. S.; Chen, F. R.; Chu, Y. S.; Yeh, H. I.; Yang, Y. C.; Yang, C. S.; Zhang, G. L.; Je, J. H.; Margaritondo, G. | |
| 2013 | |
| Source Publication | BIOTECHNOLOGY ADVANCES
 |
| ISSN | 0734-9750 |
| Volume | 31Issue:3Pages:CONCATENATE(Sheet1!I159,-Sheet1!J159) |
| Abstract | Our experimental results demonstrate that full-field hard-X-ray microscopy is finally able to investigate the internal structure of cells in tissues. This result was made possible by three main factors: the use of a coherent (synchrotron) source of X-rays, the exploitation of contrast mechanisms based on the real part of the refractive index and the magnification provided by high-resolution Fresnel zone-plate objectives. We specifically obtained high-quality microradiographs of human and mouse cells with 29 nm Rayleigh spatial resolution and verified that tomographic reconstruction could be implemented with a final resolution level suitable for subcellular features. We also demonstrated that a phase retrieval method based on a wave propagation algorithm could yield good subcellular images starting from a series of defocused microradiographs. The concluding discussion compares cellular and subcellular hard-X-ray microradiology with other techniques and evaluates its potential impact on biomedical research. (C) 2012 Elsevier Inc. All rights reserved. |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 应物所项目组 |
| WOS ID | WOS:000317441900004 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/13775 |
| Collection | 中科院上海应用物理研究所2011-2020年 |
| Recommended Citation GB/T 7714 | Chien, C. C.,Tseng, P. Y.,Chen, H. H.,et al. Imaging cells and sub-cellular structures with ultrahigh resolution full-field X-ray microscopy[J]. BIOTECHNOLOGY ADVANCES,2013,31(3):CONCATENATE(Sheet1!I159,-Sheet1!J159). |
| APA | Chien, C. C..,Tseng, P. Y..,Chen, H. H..,Hua, T. E..,Chen, S. T..,...&Margaritondo, G..(2013).Imaging cells and sub-cellular structures with ultrahigh resolution full-field X-ray microscopy.BIOTECHNOLOGY ADVANCES,31(3),CONCATENATE(Sheet1!I159,-Sheet1!J159). |
| MLA | Chien, C. C.,et al."Imaging cells and sub-cellular structures with ultrahigh resolution full-field X-ray microscopy".BIOTECHNOLOGY ADVANCES 31.3(2013):CONCATENATE(Sheet1!I159,-Sheet1!J159). |
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| Imaging cells and su（3154KB） | 开放获取 | CC BY-NC-SA | View Download | |||
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