CAS OpenIR  > 中科院上海应用物理研究所2011-2019年
Lab in a Tube: Ultrasensitive Detection of MicroRNAs at the Single-Cell Level and in Breast Cancer Patients Using Quadratic Isothermal Amplification
Duan, Ruixue; Zuo, Xiaolei; Wang, Shutao; Quan, Xiyun; Chen, Dongliang; Chen, Zhifei; Jiang, Lei; Fan, Chunhai; Xia, Fan
2013
Source PublicationJOURNAL OF THE AMERICAN CHEMICAL SOCIETY
ISSN0002-7863
Volume135Issue:12Pages:CONCATENATE(Sheet1!I188,-Sheet1!J188)
AbstractThrough rational design of a functional molecular probe with high sequence specificity that takes advantage of sensitive isothermal amplification with simple operation, we developed a one-pot hairpin-mediated quadratic enzymatic amplification strategy for microRNA (miRNA) detection. Our method exhibits ultrahigh sensitivity toward miR-21 with detection limits of 10 fM at 37 degrees C and 1 aM at 4 degrees C, which corresponds to nine strands of miR-21 in a 15 mu L sample, and it is capable of distinguishing among miRNA family members. More importantly, the proposed approach is also sensitive and selective when applied to crude extractions from MCF-7 and PC3 cell lines and even patient tissues from intraductal carcinoma and invasive ductal carcinoma of the breast.
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000317032000008
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/13800
Collection中科院上海应用物理研究所2011-2019年
Recommended Citation
GB/T 7714
Duan, Ruixue,Zuo, Xiaolei,Wang, Shutao,et al. Lab in a Tube: Ultrasensitive Detection of MicroRNAs at the Single-Cell Level and in Breast Cancer Patients Using Quadratic Isothermal Amplification[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2013,135(12):CONCATENATE(Sheet1!I188,-Sheet1!J188).
APA Duan, Ruixue.,Zuo, Xiaolei.,Wang, Shutao.,Quan, Xiyun.,Chen, Dongliang.,...&Xia, Fan.(2013).Lab in a Tube: Ultrasensitive Detection of MicroRNAs at the Single-Cell Level and in Breast Cancer Patients Using Quadratic Isothermal Amplification.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,135(12),CONCATENATE(Sheet1!I188,-Sheet1!J188).
MLA Duan, Ruixue,et al."Lab in a Tube: Ultrasensitive Detection of MicroRNAs at the Single-Cell Level and in Breast Cancer Patients Using Quadratic Isothermal Amplification".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 135.12(2013):CONCATENATE(Sheet1!I188,-Sheet1!J188).
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