Knowledge Management System Of Shanghai Institute of Applied Physics, CAS
| Mechanical force-induced DNA damage during AFM single-molecule manipulation | |
| Zhang Chen; Duan Na; Dai Bin; Zhang Yi; Zhang Donghua; Hu Jun | |
| 2013 | |
| Source Publication | NUCLEAR SCIENCE AND TECHNIQUES
 |
| ISSN | 1001-8042; 2210-3147 |
| Volume | 24Issue:3 |
| Abstract | Many environmental factors can cause DNA damage, such as radiation, heat, oxygen free radical, etc., which can induce mutation during DNA replication. Meanwhile, DNA molecules are subjected to various mechanical forces in numerous biological processes. However, it is unknown whether the mechanical force would induce DNA damage and introduce mutation during DNA replication. With the combination of single-molecule manipulation based on atomic force microscopy (AFM), single molecular polymerase chain reaction (SM-PCR) and Sanger's sequencing, we investigated the effect of mechanical force on DNA. The results show that mechanical force can cause DNA damage and induce DNA mutation during amplification. |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 应物所项目组 |
| WOS ID | WOS:000328921800011 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/13816 |
| Collection | 中科院上海应用物理研究所2011-2020年 |
| Recommended Citation GB/T 7714 | Zhang Chen,Duan Na,Dai Bin,et al. Mechanical force-induced DNA damage during AFM single-molecule manipulation[J]. NUCLEAR SCIENCE AND TECHNIQUES,2013,24(3). |
| APA | Zhang Chen,Duan Na,Dai Bin,Zhang Yi,Zhang Donghua,&Hu Jun.(2013).Mechanical force-induced DNA damage during AFM single-molecule manipulation.NUCLEAR SCIENCE AND TECHNIQUES,24(3). |
| MLA | Zhang Chen,et al."Mechanical force-induced DNA damage during AFM single-molecule manipulation".NUCLEAR SCIENCE AND TECHNIQUES 24.3(2013). |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| Mechanical force-ind（524KB） | 开放获取 | CC BY-NC-SA | View Application Full Text | |||
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