Knowledge Management System Of Shanghai Institute of Applied Physics, CAS
| Molecular Threading and Tunable Molecular Recognition on DNA Origami Nanostructures | |
| Wu, Na; Czajkowsky, Daniel M.; Zhang, Jinjin; Qu, Jianxun; Ye, Ming; Zeng, Dongdong; Zhou, Xingfei; Hu, Jun; Shao, Zhifeng; Li, Bin; Fan, Chunhai | |
| 2013 | |
| Source Publication | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
 |
| ISSN | 0002-7863 |
| Volume | 135Issue:33Pages:CONCATENATE(Sheet1!I219,-Sheet1!J219) |
| Abstract | The DNA origami technology holds great promise for the assembly of nanoscopic technological devices and studies of biochemical reactions at the single-molecule level. For these, it is essential to establish well controlled attachment of functional materials to predefined sites on the DNA origami nanostructures for reliable measurements and versatile applications. However, the two-sided nature of the origami scaffold has shown limitations in this regard. We hypothesized that holes of the commonly used two-dimensional DNA origami designs are large enough for the passage of single-stranded (ss)-DNA. Sufficiently long ssDNA initially located on one side of the origami should thus be able to "thread" to the other side through the holes in the origami sheet. By using an origami sheet attached with patterned biotinylated ssDNA spacers and monitoring streptavidin binding with atomic force microscopic (AFM) imaging, we provide unambiguous evidence that the biotin ligands positioned on one side have indeed threaded through to the other side. Our finding reveals a previously overlooked critical design feature that should provide new interpretations to previous experiments and new opportunities for the construction of origami structures with new functional capabilities. |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 应物所项目组 |
| WOS ID | WOS:000323536100006 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/13828 |
| Collection | 中科院上海应用物理研究所2011-2020年 |
| Recommended Citation GB/T 7714 | Wu, Na,Czajkowsky, Daniel M.,Zhang, Jinjin,et al. Molecular Threading and Tunable Molecular Recognition on DNA Origami Nanostructures[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2013,135(33):CONCATENATE(Sheet1!I219,-Sheet1!J219). |
| APA | Wu, Na.,Czajkowsky, Daniel M..,Zhang, Jinjin.,Qu, Jianxun.,Ye, Ming.,...&Fan, Chunhai.(2013).Molecular Threading and Tunable Molecular Recognition on DNA Origami Nanostructures.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,135(33),CONCATENATE(Sheet1!I219,-Sheet1!J219). |
| MLA | Wu, Na,et al."Molecular Threading and Tunable Molecular Recognition on DNA Origami Nanostructures".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 135.33(2013):CONCATENATE(Sheet1!I219,-Sheet1!J219). |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| Molecular Threading （3883KB） | 开放获取 | CC BY-NC-SA | View Application Full Text | |||
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment