CAS OpenIR  > 中科院上海应用物理研究所2011-2020年
Molecular Threading and Tunable Molecular Recognition on DNA Origami Nanostructures
Wu, Na; Czajkowsky, Daniel M.; Zhang, Jinjin; Qu, Jianxun; Ye, Ming; Zeng, Dongdong; Zhou, Xingfei; Hu, Jun; Shao, Zhifeng; Li, Bin; Fan, Chunhai
2013
Source PublicationJOURNAL OF THE AMERICAN CHEMICAL SOCIETY
ISSN0002-7863
Volume135Issue:33Pages:CONCATENATE(Sheet1!I219,-Sheet1!J219)
AbstractThe DNA origami technology holds great promise for the assembly of nanoscopic technological devices and studies of biochemical reactions at the single-molecule level. For these, it is essential to establish well controlled attachment of functional materials to predefined sites on the DNA origami nanostructures for reliable measurements and versatile applications. However, the two-sided nature of the origami scaffold has shown limitations in this regard. We hypothesized that holes of the commonly used two-dimensional DNA origami designs are large enough for the passage of single-stranded (ss)-DNA. Sufficiently long ssDNA initially located on one side of the origami should thus be able to "thread" to the other side through the holes in the origami sheet. By using an origami sheet attached with patterned biotinylated ssDNA spacers and monitoring streptavidin binding with atomic force microscopic (AFM) imaging, we provide unambiguous evidence that the biotin ligands positioned on one side have indeed threaded through to the other side. Our finding reveals a previously overlooked critical design feature that should provide new interpretations to previous experiments and new opportunities for the construction of origami structures with new functional capabilities.
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000323536100006
Citation statistics
Cited Times:38[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/13828
Collection中科院上海应用物理研究所2011-2020年
Recommended Citation
GB/T 7714
Wu, Na,Czajkowsky, Daniel M.,Zhang, Jinjin,et al. Molecular Threading and Tunable Molecular Recognition on DNA Origami Nanostructures[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2013,135(33):CONCATENATE(Sheet1!I219,-Sheet1!J219).
APA Wu, Na.,Czajkowsky, Daniel M..,Zhang, Jinjin.,Qu, Jianxun.,Ye, Ming.,...&Fan, Chunhai.(2013).Molecular Threading and Tunable Molecular Recognition on DNA Origami Nanostructures.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,135(33),CONCATENATE(Sheet1!I219,-Sheet1!J219).
MLA Wu, Na,et al."Molecular Threading and Tunable Molecular Recognition on DNA Origami Nanostructures".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 135.33(2013):CONCATENATE(Sheet1!I219,-Sheet1!J219).
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