CAS OpenIR  > 中科院上海应用物理研究所2011-2019年
Nanomaterial-Based Fluorescent DNA Analysis: A Comparative Study of the Quenching Effects of Graphene Oxide, Carbon Nanotubes, and Gold Nanoparticles
Li, Fan; Pei, Hao; Wang, Lihua; Lu, Jianxin; Gao, Jimin; Jiang, Bowei; Zhao, Xingchun; Fan, Chunhai
2013
Source PublicationADVANCED FUNCTIONAL MATERIALS
ISSN1616-301X; 1616-3028
Volume23Issue:33Pages:CONCATENATE(Sheet1!I224,-Sheet1!J224)
AbstractA variety of nanomaterials have shown extraordinarily high quenching ability toward a broad range of fluorophores. Recently, there has been intense interest in developing new tools for fluorescent DNA analysis in solution or inside the cell based on this property, and by exploiting interactions between these nanoscale superquenchers and DNA molecules in the single-stranded (ss-) or double-stranded (ds-) forms. Here, a comparative study on the nanoqueching effects is performed by using a series of nanomaterials with different dimensions, i.e., gold nanoparticles (AuNPs, 0D), carbon nanotubes (CNTs, 1D), and graphene oxide (GO, 2D). The quenching efficiency, kinetics, differentiation ability, and influencing factors such as concentration and ionic strength are studied. Interestingly, GO exhibits superior quenching abilities to the other two materials in both the quenching efficiency and kinetics. As a result, a GO-based fluorescent sensor, designed in a simple mix-and-detect format, can detect concentrations of DNA as low as 0.2 nM, which is better than either CNTs or AuNPs by an order of magnitude. This sensor can also differentiate single-base mismatches much better than either CNTs- or AuNPs- based sensors. This study paves the way to better choice of nanomaterials for bioanalysis and elaborate design of biosensors for both in vitro diagnosis and in vivo bioimaging.
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000327492300011
Citation statistics
Cited Times:122[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/13831
Collection中科院上海应用物理研究所2011-2019年
Recommended Citation
GB/T 7714
Li, Fan,Pei, Hao,Wang, Lihua,et al. Nanomaterial-Based Fluorescent DNA Analysis: A Comparative Study of the Quenching Effects of Graphene Oxide, Carbon Nanotubes, and Gold Nanoparticles[J]. ADVANCED FUNCTIONAL MATERIALS,2013,23(33):CONCATENATE(Sheet1!I224,-Sheet1!J224).
APA Li, Fan.,Pei, Hao.,Wang, Lihua.,Lu, Jianxin.,Gao, Jimin.,...&Fan, Chunhai.(2013).Nanomaterial-Based Fluorescent DNA Analysis: A Comparative Study of the Quenching Effects of Graphene Oxide, Carbon Nanotubes, and Gold Nanoparticles.ADVANCED FUNCTIONAL MATERIALS,23(33),CONCATENATE(Sheet1!I224,-Sheet1!J224).
MLA Li, Fan,et al."Nanomaterial-Based Fluorescent DNA Analysis: A Comparative Study of the Quenching Effects of Graphene Oxide, Carbon Nanotubes, and Gold Nanoparticles".ADVANCED FUNCTIONAL MATERIALS 23.33(2013):CONCATENATE(Sheet1!I224,-Sheet1!J224).
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