CAS OpenIR  > 中科院上海应用物理研究所2011-2020年
Probing tethered targets of a single biomolecular complex with atomic force microscopy
Wu, Na; Wang, Qi; Zhou, Xingfei; Jia, Si Si; Fan, Youjie; Hu, Jun; Li, Bin
2013
Source PublicationJOURNAL OF MOLECULAR RECOGNITION
ISSN0952-3499; 1099-1352
Volume26Issue:12Pages:CONCATENATE(Sheet1!I264,-Sheet1!J264)
AbstractDNA origami shows tremendous promise as templates for the assembly of nano-components and detection of molecular recognition events. So far, the method of choice for evaluating these structures has been atomic force microscopy (AFM), a powerful tool for imaging nanoscale objects. In most cases, tethered targets on DNA origami have proven to be highly effective samples for investigation. Still, while maximal assembly of the nanostructures might benefit from the greatest flexibility in the tether, AFM imaging requires a sufficient stability of the adsorbed components. The balance between the tether flexibility and sample stability is a major, poorly understood, concern in such studies. Here, we investigated the dependence of the tethering length on molecular capture events monitored by AFM. In our experiments, single biotin molecules were attached to DNA origami templates with various linker lengths of thymidine nucleotides, and their interaction with streptavidin was observed with AFM. Our results show that the streptavidin-biotin complexes are easily detected with short tethered lengths, and that their morphological features clearly change with the tethering length. We identify the functionally useful tether lengths for these investigations, which are also expected to prove useful in the construction and further application of DNA origami in bio-nanotechnology studies. Copyright (c) 2013 John Wiley & Sons, Ltd.
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000327375400013
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/13867
Collection中科院上海应用物理研究所2011-2020年
Recommended Citation
GB/T 7714
Wu, Na,Wang, Qi,Zhou, Xingfei,et al. Probing tethered targets of a single biomolecular complex with atomic force microscopy[J]. JOURNAL OF MOLECULAR RECOGNITION,2013,26(12):CONCATENATE(Sheet1!I264,-Sheet1!J264).
APA Wu, Na.,Wang, Qi.,Zhou, Xingfei.,Jia, Si Si.,Fan, Youjie.,...&Li, Bin.(2013).Probing tethered targets of a single biomolecular complex with atomic force microscopy.JOURNAL OF MOLECULAR RECOGNITION,26(12),CONCATENATE(Sheet1!I264,-Sheet1!J264).
MLA Wu, Na,et al."Probing tethered targets of a single biomolecular complex with atomic force microscopy".JOURNAL OF MOLECULAR RECOGNITION 26.12(2013):CONCATENATE(Sheet1!I264,-Sheet1!J264).
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