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Real Time in Vitro Regulation of DNA Methylation Using a 5-Fluorouracil Conjugated DNA-Based Stimuli-Responsive Platform
Mao, Xiuhai; Wei, Ming; Zhu, Chengfeng; Lu, Jianxin; Gao, Jimin; Simon, Anna J.; Shi, Jiye; Huang, Qing; Fan, Chunhai
2013
Source PublicationACS APPLIED MATERIALS & INTERFACES
ISSN1944-8244
Volume5Issue:7Pages:CONCATENATE(Sheet1!I279,-Sheet1!J279)
AbstractDNA methylation, catalyzed by methylases, plays a critical role in many biological processes, and many methylases have been regarded as promising targets for antimicrobial drugs. In this work, we report a stimulus responsive, self-regulating anticancer drug release platform, comprising a multifunctional DNA that upon methylation by methyltransferase (MTase) releases 5-fluorouracil (5-Fu) and in turn inhibits subsequent expression of MTase. The multifunctional DNA with anticancer drug are first methylated by DNA adenine methylation (DAM) methyltransferase (MTase) and then cut by the methylation-sensitive restriction endonuclease Dpn I. Removal of duplex from the functional DNA by the methylation/cleavage process will release the anticancer drug, resulting in inhibition of the activity of DAM in turn. Consequently, the enzyme activity of DAM MTase can be self-regulated. Furthermore, we found that the inhibition efficiency of 5-Fu significantly increase as it is functionalized with DNA.
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000317549100043
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/13882
Collection中科院上海应用物理研究所2011-2019年
Recommended Citation
GB/T 7714
Mao, Xiuhai,Wei, Ming,Zhu, Chengfeng,et al. Real Time in Vitro Regulation of DNA Methylation Using a 5-Fluorouracil Conjugated DNA-Based Stimuli-Responsive Platform[J]. ACS APPLIED MATERIALS & INTERFACES,2013,5(7):CONCATENATE(Sheet1!I279,-Sheet1!J279).
APA Mao, Xiuhai.,Wei, Ming.,Zhu, Chengfeng.,Lu, Jianxin.,Gao, Jimin.,...&Fan, Chunhai.(2013).Real Time in Vitro Regulation of DNA Methylation Using a 5-Fluorouracil Conjugated DNA-Based Stimuli-Responsive Platform.ACS APPLIED MATERIALS & INTERFACES,5(7),CONCATENATE(Sheet1!I279,-Sheet1!J279).
MLA Mao, Xiuhai,et al."Real Time in Vitro Regulation of DNA Methylation Using a 5-Fluorouracil Conjugated DNA-Based Stimuli-Responsive Platform".ACS APPLIED MATERIALS & INTERFACES 5.7(2013):CONCATENATE(Sheet1!I279,-Sheet1!J279).
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