CAS OpenIR  > 中科院上海应用物理研究所2011-2019年
Robust and efficient direct multiplex amplification method for large-scale DNA detection of blood samples on FTA cards
Jiang Bowei; Xiang Fawei; Zhao Xingchun; Wang Lihua; Fan Chunhai
2013
Source PublicationNUCLEAR SCIENCE AND TECHNIQUES
ISSN1001-8042; 2210-3147
Volume24Issue:3
AbstractDeoxyribonucleic acid (DNA) damage arising from radiations widely occurred along with the development of nuclear weapons and clinically wide application of computed tomography (CT) scan and nuclear medicine. All ionizing radiations (X-rays, gamma-rays, alpha particles, etc.) and ultraviolet (UV) radiation lead to the DNA damage. Polymerase chain reaction (PCR) is one of the most wildly used techniques for detecting DNA damage as the amplification stops at the site of the damage. Improvements to enhance the efficiency of PCR are always required and remain a great challenge. Here we establish a multiplex PCR assay system (MPAS) that is served as a robust and efficient method for direct detection of target DNA sequences in genomic DNA. The establishment of the system is performed by adding a combination of PCR enhancers to standard PCR buffer.. The performance of MPAS was demonstrated by carrying out the direct PCR amplification on 1.2 mm human blood punch using commercially available primer sets which include multiple primer pairs. The optimized PCR system resulted in high quality genotyping results without any inhibitory effect indicated and led to a full-profile success rate of 98.13%. Our studies demonstrate that the MPAS provides an efficient and robust method for obtaining sensitive, reliable and reproducible PCR results from human blood samples.
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000328921800012
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/13886
Collection中科院上海应用物理研究所2011-2019年
Recommended Citation
GB/T 7714
Jiang Bowei,Xiang Fawei,Zhao Xingchun,et al. Robust and efficient direct multiplex amplification method for large-scale DNA detection of blood samples on FTA cards[J]. NUCLEAR SCIENCE AND TECHNIQUES,2013,24(3).
APA Jiang Bowei,Xiang Fawei,Zhao Xingchun,Wang Lihua,&Fan Chunhai.(2013).Robust and efficient direct multiplex amplification method for large-scale DNA detection of blood samples on FTA cards.NUCLEAR SCIENCE AND TECHNIQUES,24(3).
MLA Jiang Bowei,et al."Robust and efficient direct multiplex amplification method for large-scale DNA detection of blood samples on FTA cards".NUCLEAR SCIENCE AND TECHNIQUES 24.3(2013).
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