CAS OpenIR  > 中科院上海应用物理研究所2011-2019年
Salts drive controllable multilayered upright assembly of amyloid-like peptides at mica/water interface
Dai, Bin; Kang, Seung-gu; Tien Huynh; Lei, Haozhi; Castelli, Matteo; Hu, Jun; Zhang, Yi; Zhou, Ruhong
2013
Source PublicationPROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
ISSN0027-8424
Volume110Issue:21Pages:CONCATENATE(Sheet1!I290,-Sheet1!J290)
AbstractSurface-assisted self-assembly of amyloid-like peptides has received considerable interest in both amyloidosis research and nanotechnology in recent years. Despite extensive studies, some controlling factors, such as salts, are still not well understood, even though it is known that some salts can promote peptide self-assemblies through the so-called "salting-out" effect. However, they are usually noncontrollable, disordered, amorphous aggregates. Here, we show via a combined experimental and theoretical approach that a conserved consensus peptide NH2-VGGAVVAGV-CONH2 (GAV-9) (from representative amyloidogenic proteins) can self-assemble into highly ordered, multilayered nanofilaments, with surprising all-upright conformations, under high-salt concentrations. Our atomic force microscopy images also demonstrate that the vertical stacking of multiple layers is highly controllable by tuning the ionic strength, such as from 0 mM (monolayer) to 100 mM (mainly double layer), and to 250 mM MgCl2 (double, triple, quadruple, and quintuple layers). Our atomistic molecular dynamics simulations then reveal that these individual layers have very different internal nanostructures, with parallel beta-sheets in the first monolayer but antiparallel beta-sheets in the subsequent upper layers due to their different microenvironment. Further studies show that the growth of multilayered, all-upright nanostructures is a common phenomenon for GAV-9 at the mica/water interface, under a variety of salt types and a wide range of salt concentrations.
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000320328700056
Citation statistics
Cited Times:30[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/13892
Collection中科院上海应用物理研究所2011-2019年
Recommended Citation
GB/T 7714
Dai, Bin,Kang, Seung-gu,Tien Huynh,et al. Salts drive controllable multilayered upright assembly of amyloid-like peptides at mica/water interface[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2013,110(21):CONCATENATE(Sheet1!I290,-Sheet1!J290).
APA Dai, Bin.,Kang, Seung-gu.,Tien Huynh.,Lei, Haozhi.,Castelli, Matteo.,...&Zhou, Ruhong.(2013).Salts drive controllable multilayered upright assembly of amyloid-like peptides at mica/water interface.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,110(21),CONCATENATE(Sheet1!I290,-Sheet1!J290).
MLA Dai, Bin,et al."Salts drive controllable multilayered upright assembly of amyloid-like peptides at mica/water interface".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 110.21(2013):CONCATENATE(Sheet1!I290,-Sheet1!J290).
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