CAS OpenIR  > 中科院上海应用物理研究所2011-2019年
Single-nucleotide polymorphism genotyping using a novel multiplexed electrochemical biosensor with nonfouling surface
Liu, Gang; Lao, Ruojun; Xu, Li; Xu, Qin; Li, Lanying; Zhang, Min; Song, Shiping; Fan, Chunhai
2013
Source PublicationBIOSENSORS & BIOELECTRONICS
ISSN0956-5663
Volume42Pages:CONCATENATE(Sheet1!I303,-Sheet1!J303)
AbstractA novel electrochemical DNA biosensor for single-nucleotide polymorphism (SNP) analysis was developed. In this work, an oligonucleotide-incorporated nonfouling surface (ONS) was constructed to resist nonspecific absorption. The biosensor was developed using a 16-electrode array for high-throughput SNP analysis. The proposed strategy was primarily based on specific oligonucleotide ligation. Fully matched target DNA templated the ligation between a capture probe assembled on gold electrodes and a tandem signal probe with a biotin moiety that could capture avidin-horseradish peroxidase and sequentially generate a catalysed amperometric signal. A pre-core mutation in the hepatitis B virus (HBV) genome at G1896A and two adjacent polymorphisms in the human CYP2C19 genome at C680T and G681A were analysed. Polymerase chain reaction (PCR) products were used as real-life samples and analysed. Our results showed that 10% of a single-mismatched mutant gene was clearly distinguished with a current signal 16 times higher than that of the blank sample, demonstrating the selectivity and practicability of the multiplexed electrochemical DNA biosensor. (C) 2012 Elsevier B.V. All rights reserved.
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000319951700085
Citation statistics
Cited Times:24[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/13903
Collection中科院上海应用物理研究所2011-2019年
Recommended Citation
GB/T 7714
Liu, Gang,Lao, Ruojun,Xu, Li,et al. Single-nucleotide polymorphism genotyping using a novel multiplexed electrochemical biosensor with nonfouling surface[J]. BIOSENSORS & BIOELECTRONICS,2013,42:CONCATENATE(Sheet1!I303,-Sheet1!J303).
APA Liu, Gang.,Lao, Ruojun.,Xu, Li.,Xu, Qin.,Li, Lanying.,...&Fan, Chunhai.(2013).Single-nucleotide polymorphism genotyping using a novel multiplexed electrochemical biosensor with nonfouling surface.BIOSENSORS & BIOELECTRONICS,42,CONCATENATE(Sheet1!I303,-Sheet1!J303).
MLA Liu, Gang,et al."Single-nucleotide polymorphism genotyping using a novel multiplexed electrochemical biosensor with nonfouling surface".BIOSENSORS & BIOELECTRONICS 42(2013):CONCATENATE(Sheet1!I303,-Sheet1!J303).
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