Knowledge Management System Of Shanghai Institute of Applied Physics, CAS
| Single-Step Rapid Assembly of DNA Origami Nanostructures for Addressable Nanoscale Bioreactors | |
| Fu, Yanming; Zeng, Dongdong; Chao, Jie; Jin, Yanqiu; Zhang, Zhao; Liu, Huajie; Li, Di; Ma, Hongwei; Huang, Qing; Gothelf, Kurt V.; Fan, Chunhai | |
| 2013 | |
| Source Publication | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
 |
| ISSN | 0002-7863 |
| Volume | 135Issue:2Pages:CONCATENATE(Sheet1!I304,-Sheet1!J304) |
| Abstract | Self-assembled DNA origami nanostructures have shown great promise for bottom-up construction of complex objects with nanoscale addressability. Here we show that DNA origami-based ID nanoribbons and nanotubes are one-pot assembled with controllable sizes and nanoscale addressability with high speed (within only 10-20 min), exhibiting extraordinarily high cooperativity that is often observed in assembly of natural molecular machines in cells (e.g. ribosome). By exploiting the high specificity of DNA-based self-assembly, we can precisely anchor proteins on these DNA origami nanostructures with sub-10 nm resolution and at the single-molecule level. We attach a pair of enzymes (horseradish peroxidase and glucose oxidase) at the inner side of DNA nanotubes and observe high coupling efficiency of enzyme cascade within this confined nanospace. Hence, DNA nanostructures with such unprecedented properties shed new light on the design of nanoscale bioreactors and nanomedicine and provide an artificial system for studying enzyme activities and cascade in highly organized and crowded cell-mimicking environments. |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 应物所项目组 |
| WOS ID | WOS:000313920800037 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/13904 |
| Collection | 中科院上海应用物理研究所2011-2020年 |
| Recommended Citation GB/T 7714 | Fu, Yanming,Zeng, Dongdong,Chao, Jie,et al. Single-Step Rapid Assembly of DNA Origami Nanostructures for Addressable Nanoscale Bioreactors[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2013,135(2):CONCATENATE(Sheet1!I304,-Sheet1!J304). |
| APA | Fu, Yanming.,Zeng, Dongdong.,Chao, Jie.,Jin, Yanqiu.,Zhang, Zhao.,...&Fan, Chunhai.(2013).Single-Step Rapid Assembly of DNA Origami Nanostructures for Addressable Nanoscale Bioreactors.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,135(2),CONCATENATE(Sheet1!I304,-Sheet1!J304). |
| MLA | Fu, Yanming,et al."Single-Step Rapid Assembly of DNA Origami Nanostructures for Addressable Nanoscale Bioreactors".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 135.2(2013):CONCATENATE(Sheet1!I304,-Sheet1!J304). |
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| File Name/Size | DocType | Version | Access | License | ||
| Single-Step Rapid As（2315KB） | 开放获取 | CC BY-NC-SA | View Download | |||
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