CAS OpenIR  > 中科院上海应用物理研究所2011-2020年
Single-Step Rapid Assembly of DNA Origami Nanostructures for Addressable Nanoscale Bioreactors
Fu, Yanming; Zeng, Dongdong; Chao, Jie; Jin, Yanqiu; Zhang, Zhao; Liu, Huajie; Li, Di; Ma, Hongwei; Huang, Qing; Gothelf, Kurt V.; Fan, Chunhai
2013
Source PublicationJOURNAL OF THE AMERICAN CHEMICAL SOCIETY
ISSN0002-7863
Volume135Issue:2Pages:CONCATENATE(Sheet1!I304,-Sheet1!J304)
AbstractSelf-assembled DNA origami nanostructures have shown great promise for bottom-up construction of complex objects with nanoscale addressability. Here we show that DNA origami-based ID nanoribbons and nanotubes are one-pot assembled with controllable sizes and nanoscale addressability with high speed (within only 10-20 min), exhibiting extraordinarily high cooperativity that is often observed in assembly of natural molecular machines in cells (e.g. ribosome). By exploiting the high specificity of DNA-based self-assembly, we can precisely anchor proteins on these DNA origami nanostructures with sub-10 nm resolution and at the single-molecule level. We attach a pair of enzymes (horseradish peroxidase and glucose oxidase) at the inner side of DNA nanotubes and observe high coupling efficiency of enzyme cascade within this confined nanospace. Hence, DNA nanostructures with such unprecedented properties shed new light on the design of nanoscale bioreactors and nanomedicine and provide an artificial system for studying enzyme activities and cascade in highly organized and crowded cell-mimicking environments.
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000313920800037
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/13904
Collection中科院上海应用物理研究所2011-2020年
Recommended Citation
GB/T 7714
Fu, Yanming,Zeng, Dongdong,Chao, Jie,et al. Single-Step Rapid Assembly of DNA Origami Nanostructures for Addressable Nanoscale Bioreactors[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2013,135(2):CONCATENATE(Sheet1!I304,-Sheet1!J304).
APA Fu, Yanming.,Zeng, Dongdong.,Chao, Jie.,Jin, Yanqiu.,Zhang, Zhao.,...&Fan, Chunhai.(2013).Single-Step Rapid Assembly of DNA Origami Nanostructures for Addressable Nanoscale Bioreactors.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,135(2),CONCATENATE(Sheet1!I304,-Sheet1!J304).
MLA Fu, Yanming,et al."Single-Step Rapid Assembly of DNA Origami Nanostructures for Addressable Nanoscale Bioreactors".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 135.2(2013):CONCATENATE(Sheet1!I304,-Sheet1!J304).
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