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| Structural Insights into the Mechanism for Recognizing Substrate of the Cytochrome P450 Enzyme TxtE | |
| Yu, Feng; Li, Minjun; Xu, Chunyan; Wang, Zhijun; Zhou, Huan; Yang, Min; Chen, Yaxing; Tang, Lin; He, Jianhua | |
| 2013 | |
| Source Publication | PLOS ONE
 |
| ISSN | 1932-6203 |
| Volume | 8Issue:11 |
| Abstract | Thaxtomins, a family of phytotoxins produced by Streptomyces spp., can cause dramatic plant cell hypertrophy and seedling stunting. Thaxtomin A is the dominant form from Streptomyces scabies and has demonstrated herbicidal action. TxtE, a cytochrome P450 enzyme from Streptomyces scabies 87.22, catalyzes direct nitration of the indolyl moiety of L-tryptophan to L-4-nitrotryptophan using nitric oxide, dioxygen and NADPH. The crystal structure of TxtE was determined at 2.1 angstrom resolution and described in this work. A clearly defined substrate access channel is observed and can be classified as channel 2a, which is common in bacteria cytochrome P450 enzymes. A continuous hydrogen bond chain from the active site to the external solvent is observed. Compared with other cytochrome P450 enzymes, TxtE shows a unique proton transfer pathway which crosses the helix I distortion. Polar contacts of Arg59, Tyr89, Asn293, Thr296, and Glu394 with L-tryptophan are seen using molecular docking analysis, which are potentially important for substrate recognition and binding. After mutating Arg59, Asn293, Thr296 or Glu394 to leucine, the substrate binding ability of TxtE was lost or decreased significantly. Based on the docking and mutation results, a possible mechanism for substrate recognition and binding is proposed. |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 应物所项目组 |
| WOS ID | WOS:000327543500106 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/13919 |
| Collection | 中科院上海应用物理研究所2011-2019年 |
| Recommended Citation GB/T 7714 | Yu, Feng,Li, Minjun,Xu, Chunyan,et al. Structural Insights into the Mechanism for Recognizing Substrate of the Cytochrome P450 Enzyme TxtE[J]. PLOS ONE,2013,8(11). |
| APA | Yu, Feng.,Li, Minjun.,Xu, Chunyan.,Wang, Zhijun.,Zhou, Huan.,...&He, Jianhua.(2013).Structural Insights into the Mechanism for Recognizing Substrate of the Cytochrome P450 Enzyme TxtE.PLOS ONE,8(11). |
| MLA | Yu, Feng,et al."Structural Insights into the Mechanism for Recognizing Substrate of the Cytochrome P450 Enzyme TxtE".PLOS ONE 8.11(2013). |
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| Structural Insights （2784KB） | 开放获取 | CC BY-NC-SA | View Download | |||
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