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Synchrotron radiation based STXM analysis and micro-XRF mapping of differential expression of extracellular thiol groups by Acidithiobacillus ferrooxidans grown on Fe2+ and S-0
Xia, Jin-Lan; Liu, Hong-Chang; Nie, Zhen-Yuan; Peng, An-An; Zhen, Xiang-Jun; Yang, Yun; Zhang, Xiu-Li
2013
Source PublicationJOURNAL OF MICROBIOLOGICAL METHODS
ISSN0167-7012
Volume94Issue:3Pages:CONCATENATE(Sheet1!I345,-Sheet1!J345)
AbstractThe differential expression of extracellular thiol groups by Acidithiobacillus ferrooxidans grown on substrates Fe2+ and S-0 was investigated by using synchrotron radiation based scanning transmission X-ray microscopy (STXM) imaging and microbeam X-ray fluorescence (mu-XRF) mapping. The extracellular thiol groups (SH) were first alkylated by iodoacetic acid forming Protein-SCH2COOH and then the P-SCH2COOH was marked by calcium ions forming P-SCH2COOCa. The STXM imaging and mu-XRF mapping of SH were based on analysis of SCH2COO-bonded Ca2+. The results indicated that the thiol group content of A. ferrooxidans grown on S-0 is 3.88 times to that on Fe2+. Combined with selective labeling of SH by Ca2+, the STXM imaging and mu-XRF mapping provided an in situ and rapid analysis of differential expression of extracellular thiol groups. (C) 2013 Elsevier B.V. All rights reserved.
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000324664500017
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/13943
Collection中科院上海应用物理研究所2011-2020年
Recommended Citation
GB/T 7714
Xia, Jin-Lan,Liu, Hong-Chang,Nie, Zhen-Yuan,et al. Synchrotron radiation based STXM analysis and micro-XRF mapping of differential expression of extracellular thiol groups by Acidithiobacillus ferrooxidans grown on Fe2+ and S-0[J]. JOURNAL OF MICROBIOLOGICAL METHODS,2013,94(3):CONCATENATE(Sheet1!I345,-Sheet1!J345).
APA Xia, Jin-Lan.,Liu, Hong-Chang.,Nie, Zhen-Yuan.,Peng, An-An.,Zhen, Xiang-Jun.,...&Zhang, Xiu-Li.(2013).Synchrotron radiation based STXM analysis and micro-XRF mapping of differential expression of extracellular thiol groups by Acidithiobacillus ferrooxidans grown on Fe2+ and S-0.JOURNAL OF MICROBIOLOGICAL METHODS,94(3),CONCATENATE(Sheet1!I345,-Sheet1!J345).
MLA Xia, Jin-Lan,et al."Synchrotron radiation based STXM analysis and micro-XRF mapping of differential expression of extracellular thiol groups by Acidithiobacillus ferrooxidans grown on Fe2+ and S-0".JOURNAL OF MICROBIOLOGICAL METHODS 94.3(2013):CONCATENATE(Sheet1!I345,-Sheet1!J345).
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