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| 3D local structure around copper site of rabbit prion-related protein: Quantitative determination by XANES spectroscopy combined with multiple-scattering calculations | |
| Cui, PX; Lian, FL; Wang, Y; Wen, Y; Chu, WS; Zhao, HF; Zhang, S; Li, J; Lin, DH; Wu, ZY; chuws@ustc.edu.cn; dhlin@xmu.edu.cn; wuzy@ustc.edu.cn | |
| 2014 | |
| Source Publication | RADIATION PHYSICS AND CHEMISTRY
 |
| ISSN | 0969-806X |
| Volume | 95Pages:69—72 |
| Abstract | Prion-related protein (PrP), a cell-surface copper-binding glycoprotein, is considered to be responsible for a number of transmissible spongiform encephalopathies (TSEs). The structural conversion of PrP from the normal cellular isoform (PrPC) to the post-translationally modified form (PrPSc) is thought to be relevant to Cu2+ binding to histidine residues. Rabbits are one of the few mammalian species that appear to be resistant to TSEs, because of the structural characteristics of the rabbit prion protein (RaPrPC) itself. Here we determined the three-dimensional local structure around the C-terminal high-affinity copper-binding sites using X-ray absorption near-edge structure combined with ab initio calculations in the framework of the multiple-scattering (MS) theory. Result shows that two amino acid resides, Gln97 and Met108, and two histidine residues, His95 and His110, are involved in binding this copper(II) ion. It might help us understand the roles of copper in prion conformation conversions, and the molecular mechanisms of prion-involved diseases. (C) 2013 Elsevier Ltd. All rights reserved. |
| Keyword | X-ray-absorption Spectra Scrapie |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000329271400016 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/13988 |
| Collection | 中科院上海应用物理研究所2011-2019年 |
| Corresponding Author | chuws@ustc.edu.cn; dhlin@xmu.edu.cn; wuzy@ustc.edu.cn |
| Recommended Citation GB/T 7714 | Cui, PX,Lian, FL,Wang, Y,et al. 3D local structure around copper site of rabbit prion-related protein: Quantitative determination by XANES spectroscopy combined with multiple-scattering calculations[J]. RADIATION PHYSICS AND CHEMISTRY,2014,95:69—72. |
| APA | Cui, PX.,Lian, FL.,Wang, Y.,Wen, Y.,Chu, WS.,...&wuzy@ustc.edu.cn.(2014).3D local structure around copper site of rabbit prion-related protein: Quantitative determination by XANES spectroscopy combined with multiple-scattering calculations.RADIATION PHYSICS AND CHEMISTRY,95,69—72. |
| MLA | Cui, PX,et al."3D local structure around copper site of rabbit prion-related protein: Quantitative determination by XANES spectroscopy combined with multiple-scattering calculations".RADIATION PHYSICS AND CHEMISTRY 95(2014):69—72. |
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