CAS OpenIR  > 中科院上海应用物理研究所2011-2019年
Characterization of protein expression of Platanus pollen following exposure to gaseous pollutants and vehicle exhaust particles
Lu, SL; Ren, JJ; Hao, XJ; Liu, DY; Zhang, RC; Wu, MH; Yi, F; Lin, J; Shinich, Y; Wang, QY; senlinlv@staff.edu.cn
2014
Source PublicationAEROBIOLOGIA
ISSN0393-5965
Volume30Issue:3Pages:281—291
AbstractBeing major ornamental street trees, species of Platanus are widely planted in the Shanghai urban area. A great deal of allergenic Platanus pollen is released from the trees and suspended in the atmosphere during its flowering season, ultimately causing allergic respiratory diseases. Few papers have focused on the distribution of this type of pollen and its expression of allergenic proteins. In order to investigate any differences in protein expression in Platanus pollen following exposure to gaseous and particulate pollutants, a special apparatus was designed. Exposure condition (such as temperature, humidity, and exposure time) of Platanus pollen and gaseous pollutants can be simulated using of this apparatus. Fresh Platanus orientalis pollen, pollutant gases (NO2, SO2, NH3), and typical urban ambient particles (vehicle exhaust particles, VEPs) were mixed in this device to examine possible changes that might occur in ambient airborne urban pollen following exposure to such pollutants. Our results showed that the fresh P. orientalis pollen became swollen, and new kinds of particles could be found on the surface of the pollen grains after exposure to the pollutants. The results of SDS-PAGE showed that five protein bands with molecular weights of 17-19, 34, 61, 82, and 144 kDa, respectively, were detected and gray scale of these brands increased after the pollen exposure to gaseous pollutants. The two-dimensional gel electrophoresis analysis demonstrated that a Platanus pollen allergenic protein (Pla a1, with a molecular weight of 18 kDa) increased in abundance following exposure to pollutant gases and VEPs, implying that air pollutants may exacerbate the allergenicity of pollen.
KeywordAcerifolia Pollen Air-pollutants Mass-spectrometry Major Allergen Climate-change Identification Pollution Pla Pollinosis Atmosphere
Indexed BySCI
Language英语
WOS IDWOS:000340393500006
Citation statistics
Cited Times:20[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/14035
Collection中科院上海应用物理研究所2011-2019年
Corresponding Authorsenlinlv@staff.edu.cn
Recommended Citation
GB/T 7714
Lu, SL,Ren, JJ,Hao, XJ,et al. Characterization of protein expression of Platanus pollen following exposure to gaseous pollutants and vehicle exhaust particles[J]. AEROBIOLOGIA,2014,30(3):281—291.
APA Lu, SL.,Ren, JJ.,Hao, XJ.,Liu, DY.,Zhang, RC.,...&senlinlv@staff.edu.cn.(2014).Characterization of protein expression of Platanus pollen following exposure to gaseous pollutants and vehicle exhaust particles.AEROBIOLOGIA,30(3),281—291.
MLA Lu, SL,et al."Characterization of protein expression of Platanus pollen following exposure to gaseous pollutants and vehicle exhaust particles".AEROBIOLOGIA 30.3(2014):281—291.
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