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DNA-Conjugated Quantum Dot Nanoprobe for High-Sensitivity Fluorescent Detection of DNA and micro-RNA
Su, S; Fan, JW; Xue, B; Yuwen, LH; Liu, XF; Pan, D; Fan, CH; Wang, LH; iamlhyuwen@njupt.edu.cn; iamlhwang@njupt.edu.cn
2014
Source PublicationACS APPLIED MATERIALS & INTERFACES
ISSN1944-8244
Volume6Issue:2Pages:1152—1157
AbstractHerein, we report a convenient approach to developing quantum dots (QDs)-based nanosensors for DNA and micro-RNA (miRNA) detection. The DNA-QDs conjugate was prepared by a ligand-exchange method. Thiollabeled ssDNA is directly attached to the QD surface, leading to highly water-dispersible nanoconjugates. The DNA-QDs conjugate has the advantages of the excellent optical properties of QDs and well-Controlled recognition properties of DNA and can be used as a nanoprobe to construct a nanosensor for nucleic acid detection. With the addition of a target nucleic acid sequence, the fluorescence intensity of QDs was quenched by an organic quencher (BHQ(2)) via Forster resonance energy transfer. This nanosensor can detect as low as 1 fM DNA and 10 fM miRNA. Moreover, the QDs-based nanosensor exhibited excellent selectivity. It not only can effectively distinguish single-base-mismatched and random nucleic sequences but also can recognize pre-miRNA and mature miRNA. Therefore, the nanosensor has high application potential for disease diagnosis and biological analysis.
KeywordGraphene Nanocrystals Protein Nanoparticles Hybridization Probes Assay
Indexed BySCI
Language英语
WOS IDWOS:000330201900056
Citation statistics
Cited Times:102[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/14088
Collection中科院上海应用物理研究所2011-2019年
Corresponding Authoriamlhyuwen@njupt.edu.cn; iamlhwang@njupt.edu.cn
Recommended Citation
GB/T 7714
Su, S,Fan, JW,Xue, B,et al. DNA-Conjugated Quantum Dot Nanoprobe for High-Sensitivity Fluorescent Detection of DNA and micro-RNA[J]. ACS APPLIED MATERIALS & INTERFACES,2014,6(2):1152—1157.
APA Su, S.,Fan, JW.,Xue, B.,Yuwen, LH.,Liu, XF.,...&iamlhwang@njupt.edu.cn.(2014).DNA-Conjugated Quantum Dot Nanoprobe for High-Sensitivity Fluorescent Detection of DNA and micro-RNA.ACS APPLIED MATERIALS & INTERFACES,6(2),1152—1157.
MLA Su, S,et al."DNA-Conjugated Quantum Dot Nanoprobe for High-Sensitivity Fluorescent Detection of DNA and micro-RNA".ACS APPLIED MATERIALS & INTERFACES 6.2(2014):1152—1157.
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