Knowledge Management System Of Shanghai Institute of Applied Physics, CAS
| DNA-Conjugated Quantum Dot Nanoprobe for High-Sensitivity Fluorescent Detection of DNA and micro-RNA | |
| Su, S; Fan, JW; Xue, B; Yuwen, LH; Liu, XF; Pan, D; Fan, CH; Wang, LH; iamlhyuwen@njupt.edu.cn; iamlhwang@njupt.edu.cn | |
| 2014 | |
| Source Publication | ACS APPLIED MATERIALS & INTERFACES
 |
| ISSN | 1944-8244 |
| Volume | 6Issue:2Pages:1152—1157 |
| Abstract | Herein, we report a convenient approach to developing quantum dots (QDs)-based nanosensors for DNA and micro-RNA (miRNA) detection. The DNA-QDs conjugate was prepared by a ligand-exchange method. Thiollabeled ssDNA is directly attached to the QD surface, leading to highly water-dispersible nanoconjugates. The DNA-QDs conjugate has the advantages of the excellent optical properties of QDs and well-Controlled recognition properties of DNA and can be used as a nanoprobe to construct a nanosensor for nucleic acid detection. With the addition of a target nucleic acid sequence, the fluorescence intensity of QDs was quenched by an organic quencher (BHQ(2)) via Forster resonance energy transfer. This nanosensor can detect as low as 1 fM DNA and 10 fM miRNA. Moreover, the QDs-based nanosensor exhibited excellent selectivity. It not only can effectively distinguish single-base-mismatched and random nucleic sequences but also can recognize pre-miRNA and mature miRNA. Therefore, the nanosensor has high application potential for disease diagnosis and biological analysis. |
| Keyword | Graphene Nanocrystals Protein Nanoparticles Hybridization Probes Assay |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000330201900056 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/14088 |
| Collection | 中科院上海应用物理研究所2011-2020年 |
| Corresponding Author | iamlhyuwen@njupt.edu.cn; iamlhwang@njupt.edu.cn |
| Recommended Citation GB/T 7714 | Su, S,Fan, JW,Xue, B,et al. DNA-Conjugated Quantum Dot Nanoprobe for High-Sensitivity Fluorescent Detection of DNA and micro-RNA[J]. ACS APPLIED MATERIALS & INTERFACES,2014,6(2):1152—1157. |
| APA | Su, S.,Fan, JW.,Xue, B.,Yuwen, LH.,Liu, XF.,...&iamlhwang@njupt.edu.cn.(2014).DNA-Conjugated Quantum Dot Nanoprobe for High-Sensitivity Fluorescent Detection of DNA and micro-RNA.ACS APPLIED MATERIALS & INTERFACES,6(2),1152—1157. |
| MLA | Su, S,et al."DNA-Conjugated Quantum Dot Nanoprobe for High-Sensitivity Fluorescent Detection of DNA and micro-RNA".ACS APPLIED MATERIALS & INTERFACES 6.2(2014):1152—1157. |
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| DNA-Conjugated Quant（3012KB） | 开放获取 | CC BY-NC-SA | View Download | |||
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