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Expression, crystallization and preliminary crystallographic data analysis of PigI, a putative L-prolyl-AMP ligase from the prodigiosin synthetic pathway in Serratia
Han, N; Ran, TT; Lou, XD; Gao, YY; He, JH; Tang, L; Xu, DQ; Wang, WW; dqxu@njau.edu.cn; wwwang@njau.edu.cn
2014
Source PublicationACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
ISSN1744-3091
Volume70Pages:624—627
AbstractProdigiosin, a member of the prodiginines, is a tripyrrole red pigment synthesized by Serratia and some other microbes. A bifurcated biosynthesis pathway of prodigiosin has been proposed in Serratia in which MBC (4-methoxy- 2,2'-bipyrrole-5-carbaldehyde) and MAP (2-methyl-3-N-amyl-pyrrole) are synthesized separately and then condensed by PigC to form prodigiosin. The first step for the synthesis of MBC is the activation of l-proline by PigI, but its catalytic mechanism has remained elusive. To elucidate its mechanism, recombinant PigI was purified and crystallized. Crystals obtained by the sitting-drop method belonged to space group P1 and diffracted to 2.0 angstrom resolution, with unit-cell parameters a = 51.2, b = 62.8, c = 91.3 angstrom, alpha = 105.1, beta = 90.1, gamma = 92.2 degrees. Matthews coefficient analysis suggested two molecules in the asymmetric unit, with a V-M of 2.6 angstrom(3) Da(-1) and a solvent content of 52.69%.
KeywordCrystal-structure Protein Crystallization Bacillus-cereus Biosynthesis Marcescens Dlta Cell Streptomyces Methylation Apoptosis
Indexed BySCI
Language英语
WOS IDWOS:000335921500017
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/14135
Collection中科院上海应用物理研究所2011-2019年
Corresponding Authordqxu@njau.edu.cn; wwwang@njau.edu.cn
Recommended Citation
GB/T 7714
Han, N,Ran, TT,Lou, XD,et al. Expression, crystallization and preliminary crystallographic data analysis of PigI, a putative L-prolyl-AMP ligase from the prodigiosin synthetic pathway in Serratia[J]. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS,2014,70:624—627.
APA Han, N.,Ran, TT.,Lou, XD.,Gao, YY.,He, JH.,...&wwwang@njau.edu.cn.(2014).Expression, crystallization and preliminary crystallographic data analysis of PigI, a putative L-prolyl-AMP ligase from the prodigiosin synthetic pathway in Serratia.ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS,70,624—627.
MLA Han, N,et al."Expression, crystallization and preliminary crystallographic data analysis of PigI, a putative L-prolyl-AMP ligase from the prodigiosin synthetic pathway in Serratia".ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS 70(2014):624—627.
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