CAS OpenIR  > 中科院上海应用物理研究所2011-2019年
GInR Negatively Regulates the Transcription of the Alanine Dehydrogenase Encoding Gene ald in Amycolatopsis mediterranei U32 under Nitrogen Limited Conditions via Specific Binding to Its Major Transcription Initiation Site
Wang, Y; Li, C; Duan, N; Li, B; Ding, XM; Yao, YF; Hu, J; Zhao, GP; Wang, J; hujun@sinap.ac.cn; gpzhao@sibs.ac.cn; cmg.wangjin@gmail.com
2014
Source PublicationPLOS ONE
ISSN1932-6203
Volume9Issue:8
AbstractAmmonium assimilation is catalyzed by two enzymatic pathways, i.e., glutamine synthetase/glutamate synthase (GS/GOGAT) and alanine dehydrogenase (AlaDH) in Amycolatopsis mediterranei U32. Under nitrogen-rich conditions, the AlaDH pathway is the major route for ammonium assimilation, while the GS/GOGAT pathway takes over when the extracellular nitrogen supply is limited. The global nitrogen regulator GlnR was previously characterized to activate the transcription of the GS encoding gene glnA in response to nitrogen limitation and is demonstrated in this study as a repressor for the transcription of the AlaDH encoding gene ald, whose regulation is consistent with the switch of the ammonium assimilation pathways from AlaDH to GS/GOGAT responding to nitrogen limitation. Three transcription initiation sites (TISs) of ald were determined with primer extension assay, among which transcription from aldP2 contributed the major transcripts under nitrogen-rich conditions but was repressed to an undetectable level in response to nitrogen limitation. Through DNase I footprinting assay, two separate regions were found to be protected by GlnR within ald promoter, within which three GlnR binding sites (a1, b1 sites in region I and a2 site in region II) were defined. Interestingly, the major TIS aldP2 is located in the middle of a2 site within region II. Therefore, one may easily conclude that GlnR represses the transcription of ald via specific binding to the GlnR binding sites, which obviously blocks the transcription initiation from aldP2 and therefore reduces ald transcripts.
KeywordGenome-wide Analysis Streptomyces-coelicolor Mycobacterium-smegmatis Escherichia-coli Glnr Metabolism Protein Identification Activation Expression
Indexed BySCI
Language英语
WOS IDWOS:000341106100024
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/14163
Collection中科院上海应用物理研究所2011-2019年
Corresponding Authorhujun@sinap.ac.cn; gpzhao@sibs.ac.cn; cmg.wangjin@gmail.com
Recommended Citation
GB/T 7714
Wang, Y,Li, C,Duan, N,et al. GInR Negatively Regulates the Transcription of the Alanine Dehydrogenase Encoding Gene ald in Amycolatopsis mediterranei U32 under Nitrogen Limited Conditions via Specific Binding to Its Major Transcription Initiation Site[J]. PLOS ONE,2014,9(8).
APA Wang, Y.,Li, C.,Duan, N.,Li, B.,Ding, XM.,...&cmg.wangjin@gmail.com.(2014).GInR Negatively Regulates the Transcription of the Alanine Dehydrogenase Encoding Gene ald in Amycolatopsis mediterranei U32 under Nitrogen Limited Conditions via Specific Binding to Its Major Transcription Initiation Site.PLOS ONE,9(8).
MLA Wang, Y,et al."GInR Negatively Regulates the Transcription of the Alanine Dehydrogenase Encoding Gene ald in Amycolatopsis mediterranei U32 under Nitrogen Limited Conditions via Specific Binding to Its Major Transcription Initiation Site".PLOS ONE 9.8(2014).
Files in This Item: Download All
File Name/Size DocType Version Access License
GInR Negatively Regu(980KB) 开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wang, Y]'s Articles
[Li, C]'s Articles
[Duan, N]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wang, Y]'s Articles
[Li, C]'s Articles
[Duan, N]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wang, Y]'s Articles
[Li, C]'s Articles
[Duan, N]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: GInR Negatively Regulates the Transcription of the Alanine Dehydrogenase Encoding Gene ald in Amycolatopsis mediterranei U32 under Nitrogen Limited Conditions via Specific Binding to ...pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.