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Gold Nanoparticle-Based Enzyme-Linked Antibody-Aptamer Sandwich Assay for Detection of Salmonella Typhimurium
Wu, WH; Li, J; Pan, D; Song, SP; Rong, MG; Li, ZX; Gao, JM; Lu, JX; jxlu313@163.com
2014
Source PublicationACS APPLIED MATERIALS & INTERFACES
ISSN1944-8244
Volume6Issue:19Pages:16974—16981
AbstractEnzyme-linked immunosorbent assay (ELISA) provides a convenient means for the detection of Salmonella enterica serovar Typhimurium (STM), which is important for rapid diagnosis of foodborne pathogens. However, conventional ELISA is limited by antibody-antigen immunoreactions and suffers from poor sensitivity and tedious sample pretreatment. Therefore, development of novel ELISA remains challenging. Herein, we designed a comprehensive strategy for rapid, sensitive, and quantitative detection of STM with high specificity by gold nanoparticle-based enzyme-linked antibody-aptamer sandwich (nano-ELAAS) method. STM was captured and preconcentrated from samples with aptamer-modified magnetic particles, followed by binding with detector antibodies. Then nanoprobes carrying a large amount of reporter antibodies and horseradish peroxidase molecules were used for colorimetric signal amplification. Under the optimized reaction conditions, the nano-ELAAS assay had a quantitative detection range from 1 X 10(3) to 1 X 10(8) CFU mL(-1), a limit of detection of 1 X 10(3) CFU mL(-1), and a selectivity of >10-fold for STM in samples containing other bacteria at higher concentration with an assay time less than 3 h. In addition, the developed nanoprobes were improved in terms of detection range and/or sensitivity when compared with two commercial enzyme-labeled antibody signal reporters. Finally, the nano-ELAAS method was demonstrated to work well in milk samples, a common source of STM contamination.
KeywordMagnetoelastic Biosensors Elisa Binding Immunoassay Selection Molecule Sequence
Indexed BySCI
Language英语
WOS IDWOS:000343018200061
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/14164
Collection中科院上海应用物理研究所2011-2020年
Corresponding Authorjxlu313@163.com
Recommended Citation
GB/T 7714
Wu, WH,Li, J,Pan, D,et al. Gold Nanoparticle-Based Enzyme-Linked Antibody-Aptamer Sandwich Assay for Detection of Salmonella Typhimurium[J]. ACS APPLIED MATERIALS & INTERFACES,2014,6(19):16974—16981.
APA Wu, WH.,Li, J.,Pan, D.,Song, SP.,Rong, MG.,...&jxlu313@163.com.(2014).Gold Nanoparticle-Based Enzyme-Linked Antibody-Aptamer Sandwich Assay for Detection of Salmonella Typhimurium.ACS APPLIED MATERIALS & INTERFACES,6(19),16974—16981.
MLA Wu, WH,et al."Gold Nanoparticle-Based Enzyme-Linked Antibody-Aptamer Sandwich Assay for Detection of Salmonella Typhimurium".ACS APPLIED MATERIALS & INTERFACES 6.19(2014):16974—16981.
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