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| How water molecules affect the catalytic activity of hydrolases - A XANES study of the local structures of peptide deformylase | |
| Cui, PX; Wang, Y; Chu, WS; Guo, XY; Yang, FF; Yu, MJ; Zhao, HF; Dong, YH; Xie, YN; Gong, WM; Wu, ZY; chuws@ustc.edu.cn; wgong@sun5.ibp.ac.cn; wuzy@ustc.edu.cn | |
| 2014 | |
| Source Publication | SCIENTIFIC REPORTS
 |
| ISSN | 2045-2322 |
| Volume | 4 |
| Abstract | Peptide deformylase (PDF) is a prokaryotic enzyme that catalyzes the deformylation of nascent peptides generated during protein synthesis and water molecules play a key role in these hydrolases. Using X-ray absorption near edge spectroscopy (XANES) and ab initio calculations we accurately probe the local atomic environment of the metal ion binding in the active site of PDF at different pHvalues and with different metal ions. This new approach is an effective way to monitor existing correlations among functions and structural changes. We show for the first time that the enzymatic activity depends on pH values and metal ions via the bond length of the nearest coordinating water (Wat1) to the metal ion. Combining experimental and theoretical data we may claim that PDF exhibits an enhanced enzymatic activity only when the distance of the Wat1 molecule with the metal ion falls in the limited range from 2.15 to 2.55 angstrom. |
| Keyword | X-ray-absorption Bacterium Leptospira-interrogans Iron-molybdenum Cofactor Escherichia-coli k Edges Spectra Protein Metal Spectroscopy Insight |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000346300500006 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/14177 |
| Collection | 中科院上海应用物理研究所2011-2019年 |
| Corresponding Author | chuws@ustc.edu.cn; wgong@sun5.ibp.ac.cn; wuzy@ustc.edu.cn |
| Recommended Citation GB/T 7714 | Cui, PX,Wang, Y,Chu, WS,et al. How water molecules affect the catalytic activity of hydrolases - A XANES study of the local structures of peptide deformylase[J]. SCIENTIFIC REPORTS,2014,4. |
| APA | Cui, PX.,Wang, Y.,Chu, WS.,Guo, XY.,Yang, FF.,...&wuzy@ustc.edu.cn.(2014).How water molecules affect the catalytic activity of hydrolases - A XANES study of the local structures of peptide deformylase.SCIENTIFIC REPORTS,4. |
| MLA | Cui, PX,et al."How water molecules affect the catalytic activity of hydrolases - A XANES study of the local structures of peptide deformylase".SCIENTIFIC REPORTS 4(2014). |
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| File Name/Size | DocType | Version | Access | License | ||
| How water molecules （833KB） | 开放获取 | CC BY-NC-SA | View Application Full Text | |||
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