CAS OpenIR  > 中科院上海应用物理研究所2011-2019年
How water molecules affect the catalytic activity of hydrolases - A XANES study of the local structures of peptide deformylase
Cui, PX; Wang, Y; Chu, WS; Guo, XY; Yang, FF; Yu, MJ; Zhao, HF; Dong, YH; Xie, YN; Gong, WM; Wu, ZY; chuws@ustc.edu.cn; wgong@sun5.ibp.ac.cn; wuzy@ustc.edu.cn
2014
Source PublicationSCIENTIFIC REPORTS
ISSN2045-2322
Volume4
AbstractPeptide deformylase (PDF) is a prokaryotic enzyme that catalyzes the deformylation of nascent peptides generated during protein synthesis and water molecules play a key role in these hydrolases. Using X-ray absorption near edge spectroscopy (XANES) and ab initio calculations we accurately probe the local atomic environment of the metal ion binding in the active site of PDF at different pHvalues and with different metal ions. This new approach is an effective way to monitor existing correlations among functions and structural changes. We show for the first time that the enzymatic activity depends on pH values and metal ions via the bond length of the nearest coordinating water (Wat1) to the metal ion. Combining experimental and theoretical data we may claim that PDF exhibits an enhanced enzymatic activity only when the distance of the Wat1 molecule with the metal ion falls in the limited range from 2.15 to 2.55 angstrom.
KeywordX-ray-absorption Bacterium Leptospira-interrogans Iron-molybdenum Cofactor Escherichia-coli k Edges Spectra Protein Metal Spectroscopy Insight
Indexed BySCI
Language英语
WOS IDWOS:000346300500006
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/14177
Collection中科院上海应用物理研究所2011-2019年
Corresponding Authorchuws@ustc.edu.cn; wgong@sun5.ibp.ac.cn; wuzy@ustc.edu.cn
Recommended Citation
GB/T 7714
Cui, PX,Wang, Y,Chu, WS,et al. How water molecules affect the catalytic activity of hydrolases - A XANES study of the local structures of peptide deformylase[J]. SCIENTIFIC REPORTS,2014,4.
APA Cui, PX.,Wang, Y.,Chu, WS.,Guo, XY.,Yang, FF.,...&wuzy@ustc.edu.cn.(2014).How water molecules affect the catalytic activity of hydrolases - A XANES study of the local structures of peptide deformylase.SCIENTIFIC REPORTS,4.
MLA Cui, PX,et al."How water molecules affect the catalytic activity of hydrolases - A XANES study of the local structures of peptide deformylase".SCIENTIFIC REPORTS 4(2014).
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