Knowledge Management System Of Shanghai Institute of Applied Physics, CAS
| Hybridization Chain Reaction Amplification of MicroRNA Detection with a Tetrahedral DNA Nanostructure-Based Electrochemical Biosensor | |
| Ge, ZL; Lin, MH; Wang, P; Pei, H; Yan, J; Sho, JY; Huang, Q; He, DN; Fan, CH; Zuo, XL; zuoxiaolei@sinap.ac.cn | |
| 2014 | |
| Source Publication | ANALYTICAL CHEMISTRY
 |
| ISSN | 0003-2700 |
| Volume | 86Issue:4Pages:2124—2130 |
| Abstract | There remains a great challenge in the sensitive detection of microRNA because of the short length and low abundance of microRNAs in cells. Here, we have demonstrated an ultrasensitive detection platform for microRNA by combining the tetrahedral DNA nanostructure probes and hybridization chain reaction (HCR) amplification. The detection limits for DNA and microRNA are 100 aM and 10 aM (corresponding to 600 microRNAs in a 100 mu L sample), respectively. Compared to the widely used supersandwich amplification, the detection limits are improved by 3 orders of magnitude. The uncontrolled surface immobilization and consumption of target molecules that limit the amplification efficiency of supersandwich are eliminated in our platform. Taking advantage of DNA nanotechnology, we employed three-dimensional tetrahedral DNA nanostructure as the scaffold to immobilize DNA recognition probes to increase the reactivity and accessibility, while DNA nanowire tentacles are used for efficient signal amplification by capturing multiple catalytic enzymes in a highly ordered way. The synergetic effect of DNA tetrahedron and nanowire tentacles have proven to greatly improve sensitivity for both DNA and microRNA detection. |
| Keyword | Sequence-specific Detection Personal Glucose Meters Ultrasensitive Detection Signal Amplification Complex Matrices Targets Sensors Surfaces Theophylline Expression |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000331775600028 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/14179 |
| Collection | 中科院上海应用物理研究所2011-2020年 |
| Corresponding Author | zuoxiaolei@sinap.ac.cn |
| Recommended Citation GB/T 7714 | Ge, ZL,Lin, MH,Wang, P,et al. Hybridization Chain Reaction Amplification of MicroRNA Detection with a Tetrahedral DNA Nanostructure-Based Electrochemical Biosensor[J]. ANALYTICAL CHEMISTRY,2014,86(4):2124—2130. |
| APA | Ge, ZL.,Lin, MH.,Wang, P.,Pei, H.,Yan, J.,...&zuoxiaolei@sinap.ac.cn.(2014).Hybridization Chain Reaction Amplification of MicroRNA Detection with a Tetrahedral DNA Nanostructure-Based Electrochemical Biosensor.ANALYTICAL CHEMISTRY,86(4),2124—2130. |
| MLA | Ge, ZL,et al."Hybridization Chain Reaction Amplification of MicroRNA Detection with a Tetrahedral DNA Nanostructure-Based Electrochemical Biosensor".ANALYTICAL CHEMISTRY 86.4(2014):2124—2130. |
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| Hybridization Chain （2469KB） | 开放获取 | CC BY-NC-SA | View Download | |||
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