CAS OpenIR  > 中科院上海应用物理研究所2011-2019年
Label-Free Quantitative Detection of Tumor-Derived Exosomes through Surface Plasmon Resonance Imaging
Zhu, L; Wang, K; Cui, J; Liu, H; Bu, XL; Ma, HL; Wang, WZ; Gong, H; Lausted, C; Hood, L; Yang, G; Hu, ZY; yangguang9002@163.com; huzy@nanoctr.cn
2014
Source PublicationANALYTICAL CHEMISTRY
ISSN0003-2700
Volume86Issue:17Pages:8857—8864
AbstractExosomes are endosome-derived membrane vesicles carrying proteins and nucleic adds that are involved in cellular functions such as intercellular communication, protein and RNA secretion, and antigen presentation. Therefore, exosomes serve as potential biomarkers for many diseases including cancer. Because exosomes are difficult to enrich or purify from biofluids, quantification of exosomes is tedious and inaccurate. Here, we present a real-time, label-free, and quantitative method to detect and characterize tumor-derived exosomes without enrichment or purification. Utilizing surface plasmon resonance imaging (SPRi) in combination with antibody microarrays specific to the extracellular domains of exosome membrane proteins, exosomes in tumor cell culture medium can be quantitatively detected. We found a positive correlation between the metastatic potential of tumor cell lines and exosome secretion. This method provides an easy, efficient, and novel way to detect exosome secretion and thus an avenue toward the diagnosis and prognosis prediction of cancer.
KeywordTransferrin Receptor Rejection Antigens Proteomic Analysis Membrane-vesicles High-throughput B-lymphocytes Cell Microvesicles Cancer Metastasis
Indexed BySCI
Language英语
WOS IDWOS:000341229200050
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/14211
Collection中科院上海应用物理研究所2011-2019年
Corresponding Authoryangguang9002@163.com; huzy@nanoctr.cn
Recommended Citation
GB/T 7714
Zhu, L,Wang, K,Cui, J,et al. Label-Free Quantitative Detection of Tumor-Derived Exosomes through Surface Plasmon Resonance Imaging[J]. ANALYTICAL CHEMISTRY,2014,86(17):8857—8864.
APA Zhu, L.,Wang, K.,Cui, J.,Liu, H.,Bu, XL.,...&huzy@nanoctr.cn.(2014).Label-Free Quantitative Detection of Tumor-Derived Exosomes through Surface Plasmon Resonance Imaging.ANALYTICAL CHEMISTRY,86(17),8857—8864.
MLA Zhu, L,et al."Label-Free Quantitative Detection of Tumor-Derived Exosomes through Surface Plasmon Resonance Imaging".ANALYTICAL CHEMISTRY 86.17(2014):8857—8864.
Files in This Item: Download All
File Name/Size DocType Version Access License
Label-Free Quantitat(3010KB) 开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhu, L]'s Articles
[Wang, K]'s Articles
[Cui, J]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhu, L]'s Articles
[Wang, K]'s Articles
[Cui, J]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhu, L]'s Articles
[Wang, K]'s Articles
[Cui, J]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Label-Free Quantitative Detection of Tumor-Derived Exosomes through Surface Plasmon Resonance Imaging.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.