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| Label-Free Quantitative Detection of Tumor-Derived Exosomes through Surface Plasmon Resonance Imaging | |
| Zhu, L; Wang, K; Cui, J; Liu, H; Bu, XL; Ma, HL; Wang, WZ; Gong, H; Lausted, C; Hood, L; Yang, G; Hu, ZY; yangguang9002@163.com; huzy@nanoctr.cn | |
| 2014 | |
| Source Publication | ANALYTICAL CHEMISTRY
 |
| ISSN | 0003-2700 |
| Volume | 86Issue:17Pages:8857—8864 |
| Abstract | Exosomes are endosome-derived membrane vesicles carrying proteins and nucleic adds that are involved in cellular functions such as intercellular communication, protein and RNA secretion, and antigen presentation. Therefore, exosomes serve as potential biomarkers for many diseases including cancer. Because exosomes are difficult to enrich or purify from biofluids, quantification of exosomes is tedious and inaccurate. Here, we present a real-time, label-free, and quantitative method to detect and characterize tumor-derived exosomes without enrichment or purification. Utilizing surface plasmon resonance imaging (SPRi) in combination with antibody microarrays specific to the extracellular domains of exosome membrane proteins, exosomes in tumor cell culture medium can be quantitatively detected. We found a positive correlation between the metastatic potential of tumor cell lines and exosome secretion. This method provides an easy, efficient, and novel way to detect exosome secretion and thus an avenue toward the diagnosis and prognosis prediction of cancer. |
| Keyword | Transferrin Receptor Rejection Antigens Proteomic Analysis Membrane-vesicles High-throughput B-lymphocytes Cell Microvesicles Cancer Metastasis |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000341229200050 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/14211 |
| Collection | 中科院上海应用物理研究所2011-2019年 |
| Corresponding Author | yangguang9002@163.com; huzy@nanoctr.cn |
| Recommended Citation GB/T 7714 | Zhu, L,Wang, K,Cui, J,et al. Label-Free Quantitative Detection of Tumor-Derived Exosomes through Surface Plasmon Resonance Imaging[J]. ANALYTICAL CHEMISTRY,2014,86(17):8857—8864. |
| APA | Zhu, L.,Wang, K.,Cui, J.,Liu, H.,Bu, XL.,...&huzy@nanoctr.cn.(2014).Label-Free Quantitative Detection of Tumor-Derived Exosomes through Surface Plasmon Resonance Imaging.ANALYTICAL CHEMISTRY,86(17),8857—8864. |
| MLA | Zhu, L,et al."Label-Free Quantitative Detection of Tumor-Derived Exosomes through Surface Plasmon Resonance Imaging".ANALYTICAL CHEMISTRY 86.17(2014):8857—8864. |
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