CAS OpenIR  > 中科院上海应用物理研究所2011-2019年
Metal Ion-Mediated Assembly of DNA Nanostructures for Cascade Fluorescence Resonance Energy Transfer-Based Fingerprint Analysis
Xia, JY; Lin, MH; Zuo, XL; Su, S; Wang, LH; Huang, W; Fan, CH; Huang, Q; huangqing@sinap.ac.cn
2014
Source PublicationANALYTICAL CHEMISTRY
ISSN0003-2700
Volume86Issue:14Pages:7084—7087
AbstractContamination of heavy metal ions in an aquatic environment poses a serious threat to human health. More seriously, heavy metal ions are usually present in the environment in a mixture, and the synergetic toxicity of multiple heavy metal ions is revealed (Aragay et al. Chem. Rev. 2011, 111, 3433; Chu et al. Aquat. Toxicol. 2002, 61, 53). Unfortunately, most of the existing methods based on DNA sequences are focusing on the detection of one type of metal ions. Simple and multiplexed detection of multiple metal ions has been poorly investigated and remains challenging. Here, we re-engineered the DNA sequences for Pb2+, Hg2+, and Ag+, through which the binding of multiple metal ions initiated the self-assembly of these DNA sequences. On the basis of our rationally designed multicolor fluorescent labeling of the DNA sequences, cascade fluorescence resonance energy transfer (FRET) occurred. As a result, a fingerprint fluorescent spectrum was produced to indicate the presence of a single type of metal ions or multiple metal ions. The major advantages of our cascade FRET fingerprint technology include the following: (1) the "mix and read" detection mode in homogeneous solution is simple without the need of complicated instruments; (2) only single excitation is required to provide the cascade FRET fingerprint spectrum; (3) multiplexed detection capability can be realized intuitively and sensitively.
KeywordHg2++ Sensor Lead Mercury(Ii) Ag++
Indexed BySCI
Language英语
WOS IDWOS:000339227400050
Citation statistics
Cited Times:25[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/14246
Collection中科院上海应用物理研究所2011-2019年
Corresponding Authorhuangqing@sinap.ac.cn
Recommended Citation
GB/T 7714
Xia, JY,Lin, MH,Zuo, XL,et al. Metal Ion-Mediated Assembly of DNA Nanostructures for Cascade Fluorescence Resonance Energy Transfer-Based Fingerprint Analysis[J]. ANALYTICAL CHEMISTRY,2014,86(14):7084—7087.
APA Xia, JY.,Lin, MH.,Zuo, XL.,Su, S.,Wang, LH.,...&huangqing@sinap.ac.cn.(2014).Metal Ion-Mediated Assembly of DNA Nanostructures for Cascade Fluorescence Resonance Energy Transfer-Based Fingerprint Analysis.ANALYTICAL CHEMISTRY,86(14),7084—7087.
MLA Xia, JY,et al."Metal Ion-Mediated Assembly of DNA Nanostructures for Cascade Fluorescence Resonance Energy Transfer-Based Fingerprint Analysis".ANALYTICAL CHEMISTRY 86.14(2014):7084—7087.
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