CAS OpenIR  > 中科院上海应用物理研究所2011-2019年
Multivalent Capture and Detection of Cancer Cells with DNA Nanostructured Biosensors and Multibranched Hybridization Chain Reaction Amplification
Zhou, GB; Lin, MH; Song, P; Chen, XQ; Chao, J; Wang, LH; Huang, Q; Huang, W; Fan, CH; Zuo, XL; xqchen@csu.edu.cn; zuoxiaolei@sinap.ac.cn
2014
Source PublicationANALYTICAL CHEMISTRY
ISSN0003-2700
Volume86Issue:15Pages:7843—7848
AbstractSensitive detection of cancer cells plays a critically important role in the early detection of cancer and cancer metastasis. However, because circulating tumor cells are extremely rare in peripheral blood, the detection of cancer cells with high analytical sensitivity and specificity remains challenging. Here, we have demonstrated a simple, sensitive and specific detection of cancer cells with the detection sensitivity of four cancer cells, which is lower than the cutoff value with respect to correlation with survival outcomes as well as predictive of metastatic disease in clinical diagnostics. We re-engineered the hybridization chain reaction (HCR) to multibranched HCR (mHCR) that can produce long products with multiple biotins for signal amplification and multiple branched arms for multivalent binding. The capturing gold surface is modified with DNA tetrahedral probes, which provide superior hybridization conditions for the multivalent binding. The synergetic effect of mHCR amplification and multivalent binding lead to the high sensitivity of our detection platform.
KeywordCirculating Tumor-cells Quantum Dots Label-free Nanoparticles Expression Transport Microrna Density Probes Assay
Indexed BySCI
Language英语
WOS IDWOS:000340081100096
Citation statistics
Cited Times:98[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/14260
Collection中科院上海应用物理研究所2011-2019年
Corresponding Authorxqchen@csu.edu.cn; zuoxiaolei@sinap.ac.cn
Recommended Citation
GB/T 7714
Zhou, GB,Lin, MH,Song, P,et al. Multivalent Capture and Detection of Cancer Cells with DNA Nanostructured Biosensors and Multibranched Hybridization Chain Reaction Amplification[J]. ANALYTICAL CHEMISTRY,2014,86(15):7843—7848.
APA Zhou, GB.,Lin, MH.,Song, P.,Chen, XQ.,Chao, J.,...&zuoxiaolei@sinap.ac.cn.(2014).Multivalent Capture and Detection of Cancer Cells with DNA Nanostructured Biosensors and Multibranched Hybridization Chain Reaction Amplification.ANALYTICAL CHEMISTRY,86(15),7843—7848.
MLA Zhou, GB,et al."Multivalent Capture and Detection of Cancer Cells with DNA Nanostructured Biosensors and Multibranched Hybridization Chain Reaction Amplification".ANALYTICAL CHEMISTRY 86.15(2014):7843—7848.
Files in This Item: Download All
File Name/Size DocType Version Access License
Multivalent Capture (2645KB) 开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhou, GB]'s Articles
[Lin, MH]'s Articles
[Song, P]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhou, GB]'s Articles
[Lin, MH]'s Articles
[Song, P]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhou, GB]'s Articles
[Lin, MH]'s Articles
[Song, P]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Multivalent Capture and Detection of Cancer Cells with DNA Nanostructured Biosensors and Multibranched Hybridization Chain Reaction Amplification.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.