CAS OpenIR  > 中科院上海应用物理研究所2011-2019年
Target-Responsive, DNA Nanostructure-Based E-DNA Sensor for microRNA Analysis
Lin, MH; Wen, YL; Li, LY; Pei, H; Liu, G; Song, HY; Zuo, XL; Fan, CH; Huang, Q; huangqing@sinap.ac.cn
2014
Source PublicationANALYTICAL CHEMISTRY
ISSN0003-2700
Volume86Issue:5Pages:2285—2288
AbstractBecause of the short size and low abundance of microRNAs, it is challenging to develop fast, inexpensive, and simple biosensors to detect them. In this work, we have demonstrated a new generation (the third generation) of E-DNA sensor for the sensitive and specific detection of microRNAs. Our third generation of E-DNA sensor can sensitively detect microRNA target (microRNA-141) as low as 1 fM. The excellent specificity has been demonstrated by its differential ability to the highly similar microRNA analogues. In our design, the use of DNA tetrahedron ensures the stem-loop structure in well controlled density with improved reactivity. The regulation of the thermodynamic stability of the stem-loop structure decreases the background signal and increases the specificity as well. The enzymes attached bring the electrocatalytic signal to amplify the detection. The combination of these effects improves the sensitivity of the E-DNA sensor and makes it suitable to the microRNA detection. Finally, our third generation of E-DNA sensor is generalizable to the detection of other micro RNA targets (for example, microRNA-21).
KeywordSequence-specific Detection Electrochemical Biosensors Blood-serum Label-free Reagentless Pseudoknot
Indexed BySCI
Language英语
WOS IDWOS:000332494100004
Citation statistics
Cited Times:98[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/14463
Collection中科院上海应用物理研究所2011-2019年
Corresponding Authorhuangqing@sinap.ac.cn
Recommended Citation
GB/T 7714
Lin, MH,Wen, YL,Li, LY,et al. Target-Responsive, DNA Nanostructure-Based E-DNA Sensor for microRNA Analysis[J]. ANALYTICAL CHEMISTRY,2014,86(5):2285—2288.
APA Lin, MH.,Wen, YL.,Li, LY.,Pei, H.,Liu, G.,...&huangqing@sinap.ac.cn.(2014).Target-Responsive, DNA Nanostructure-Based E-DNA Sensor for microRNA Analysis.ANALYTICAL CHEMISTRY,86(5),2285—2288.
MLA Lin, MH,et al."Target-Responsive, DNA Nanostructure-Based E-DNA Sensor for microRNA Analysis".ANALYTICAL CHEMISTRY 86.5(2014):2285—2288.
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