Knowledge Management System Of Shanghai Institute of Applied Physics, CAS
| Target-Responsive, DNA Nanostructure-Based E-DNA Sensor for microRNA Analysis | |
| Lin, MH; Wen, YL; Li, LY; Pei, H; Liu, G; Song, HY; Zuo, XL; Fan, CH; Huang, Q; huangqing@sinap.ac.cn | |
| 2014 | |
| Source Publication | ANALYTICAL CHEMISTRY
 |
| ISSN | 0003-2700 |
| Volume | 86Issue:5Pages:2285—2288 |
| Abstract | Because of the short size and low abundance of microRNAs, it is challenging to develop fast, inexpensive, and simple biosensors to detect them. In this work, we have demonstrated a new generation (the third generation) of E-DNA sensor for the sensitive and specific detection of microRNAs. Our third generation of E-DNA sensor can sensitively detect microRNA target (microRNA-141) as low as 1 fM. The excellent specificity has been demonstrated by its differential ability to the highly similar microRNA analogues. In our design, the use of DNA tetrahedron ensures the stem-loop structure in well controlled density with improved reactivity. The regulation of the thermodynamic stability of the stem-loop structure decreases the background signal and increases the specificity as well. The enzymes attached bring the electrocatalytic signal to amplify the detection. The combination of these effects improves the sensitivity of the E-DNA sensor and makes it suitable to the microRNA detection. Finally, our third generation of E-DNA sensor is generalizable to the detection of other micro RNA targets (for example, microRNA-21). |
| Keyword | Sequence-specific Detection Electrochemical Biosensors Blood-serum Label-free Reagentless Pseudoknot |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000332494100004 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/14463 |
| Collection | 中科院上海应用物理研究所2011-2019年 |
| Corresponding Author | huangqing@sinap.ac.cn |
| Recommended Citation GB/T 7714 | Lin, MH,Wen, YL,Li, LY,et al. Target-Responsive, DNA Nanostructure-Based E-DNA Sensor for microRNA Analysis[J]. ANALYTICAL CHEMISTRY,2014,86(5):2285—2288. |
| APA | Lin, MH.,Wen, YL.,Li, LY.,Pei, H.,Liu, G.,...&huangqing@sinap.ac.cn.(2014).Target-Responsive, DNA Nanostructure-Based E-DNA Sensor for microRNA Analysis.ANALYTICAL CHEMISTRY,86(5),2285—2288. |
| MLA | Lin, MH,et al."Target-Responsive, DNA Nanostructure-Based E-DNA Sensor for microRNA Analysis".ANALYTICAL CHEMISTRY 86.5(2014):2285—2288. |
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| Target-Responsive, D（2169KB） | 开放获取 | CC BY-NC-SA | View Download | |||
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