Knowledge Management System Of Shanghai Institute of Applied Physics, CAS
| Tryptamine functionalized reduced graphene oxide for label-free DNA impedimetric biosensing | |
| Zhang, Z; Luo, LQ; Chen, GF; Ding, YP; Deng, DM; Fan, CH; luck@shu.edu.cn | |
| 2014 | |
| Source Publication | BIOSENSORS & BIOELECTRONICS
 |
| ISSN | 0956-5663 |
| Volume | 60Pages:161—166 |
| Abstract | A novel simple protocol of synthesizing tryptamine-functionalized reduced graphene oxide (TRA-rGO) was proposed to fabricate label-free electrochemical impedance DNA biosensor. TRA was anchored to rGO with its indole ring via pi-stacking interaction. The morphology, conductivity and interaction of TRA-rGO were characterized by atomic force microscopy, high resolution transmission electron microscopy, cyclic voltammetry, electrochemical impedance spectroscopy (EIS) as well as Raman and fluorescence spectroscopy. The amino-substituted oligonucleotide probe was conjugated to TRA by cross-linker glutaraldehyde for preparing an electrochemical biosensing platform. The DNA hybridization reaction of oligonucleotide probe with target DNA was monitored by EIS. Under optimum conditions, the proposed biosensor exhibited high sensitivity and low detection limit for detecting complementary oligonucleotide. The target oligonucleotide could be quantified in a wide range of 1.0 x 10(-12)-1.0 x 10(-7) M with low detection limit of 5.2 x 10(-13) M (S/N=3). (C) 2014 Elsevier B.V. All rights reserved. |
| Keyword | Electrochemical Biosensor Hybrid Materials Nanocomposites Graphite Sheets Films Amplification Exfoliation Composites Microscopy |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000337863900022 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/14530 |
| Collection | 中科院上海应用物理研究所2011-2020年 |
| Corresponding Author | luck@shu.edu.cn |
| Recommended Citation GB/T 7714 | Zhang, Z,Luo, LQ,Chen, GF,et al. Tryptamine functionalized reduced graphene oxide for label-free DNA impedimetric biosensing[J]. BIOSENSORS & BIOELECTRONICS,2014,60:161—166. |
| APA | Zhang, Z.,Luo, LQ.,Chen, GF.,Ding, YP.,Deng, DM.,...&luck@shu.edu.cn.(2014).Tryptamine functionalized reduced graphene oxide for label-free DNA impedimetric biosensing.BIOSENSORS & BIOELECTRONICS,60,161—166. |
| MLA | Zhang, Z,et al."Tryptamine functionalized reduced graphene oxide for label-free DNA impedimetric biosensing".BIOSENSORS & BIOELECTRONICS 60(2014):161—166. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| Tryptamine functiona（1430KB） | 开放获取 | CC BY-NC-SA | View Application Full Text | |||
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