CAS OpenIR  > 中科院上海应用物理研究所2011-2019年
Ultrasensitive Electrochemical Detection of Prostate-Specific Antigen by Using Antibodies Anchored on a DNA Nanostructural Scaffold
Chen, XQ; Zhou, GB; Song, P; Wang, JJ; Gao, JM; Lu, JX; Fan, CH; Zuo, XL; zuoxiaolei@sinap.ac.cn
2014
Source PublicationANALYTICAL CHEMISTRY
ISSN0003-2700
Volume86Issue:15Pages:7337—7342
AbstractThe high occurrence of prostate cancer in men makes the prostate-specific antigen (PSA) screening test really important. More importantly, the recurrence rate after radical prostatectomy is high, whereas the traditional P SA immunoassay does not possess the sufficient high sensitivity for post-treatment PSA detection. In these assays, uncontrolled and random orientation of capture antibodies on the surface largely reduces their activity. Here, by exploiting the rapidly emerging DNA nanotechnology, we developed a DNA nanostructure based scaffold to precisely control the assembly of antibody monolayer. We demonstrated that the detection sensitivity was critically dependent on the nanoscale-spacing (nanospacing) of immobilized antibodies. In addition to the controlled assembly, we further amplified the sensing signal by using the gold nanoparticles, resulting in extremely high sensitivity and a low detection limit of 1 pg/mL. To test the real-world applicability of our nanoengineered electrochemical sensor, we evaluated the performance with 11 patients' serum samples and obtained consistent results with the "gold-standard" assays.
KeywordPromising Plasma Biomarkers Assay Reveals Prostasomes Electron-transfer Binding Immobilization Surfaces Construction Proteins Sensors Cancer
Indexed BySCI
Language英语
WOS IDWOS:000340081100030
Citation statistics
Cited Times:99[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/14536
Collection中科院上海应用物理研究所2011-2019年
Corresponding Authorzuoxiaolei@sinap.ac.cn
Recommended Citation
GB/T 7714
Chen, XQ,Zhou, GB,Song, P,et al. Ultrasensitive Electrochemical Detection of Prostate-Specific Antigen by Using Antibodies Anchored on a DNA Nanostructural Scaffold[J]. ANALYTICAL CHEMISTRY,2014,86(15):7337—7342.
APA Chen, XQ.,Zhou, GB.,Song, P.,Wang, JJ.,Gao, JM.,...&zuoxiaolei@sinap.ac.cn.(2014).Ultrasensitive Electrochemical Detection of Prostate-Specific Antigen by Using Antibodies Anchored on a DNA Nanostructural Scaffold.ANALYTICAL CHEMISTRY,86(15),7337—7342.
MLA Chen, XQ,et al."Ultrasensitive Electrochemical Detection of Prostate-Specific Antigen by Using Antibodies Anchored on a DNA Nanostructural Scaffold".ANALYTICAL CHEMISTRY 86.15(2014):7337—7342.
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