CAS OpenIR  > 中科院上海应用物理研究所2011-2020年
Utilisation of adsorption and desorption for simultaneously improving protein crystallisation success rate and crystal quality
Guo, YZ; Sun, LH; Oberthuer, D; Zhang, CY; Shi, JY; Di, JL; Zhang, BL; Cao, HL; Liu, YM; Li, J; Wang, Q; Huang, HH; Liu, J; Schulz, JM; Zhang, QY; Zhao, JL; Betzel, C; He, JH; Yin, DC; Christian.Betzel@uni-hamburg.de; hejianhua@sinap.ac.cn; yindc@nwpu.edu.cn
2014
Source PublicationSCIENTIFIC REPORTS
ISSN2045-2322
Volume4
AbstractHigh-quality protein crystals of suitable size are an important prerequisite for applying X-ray crystallography to determine the 3-dimensional structure of proteins. However, it is often difficult to obtain protein crystals of appropriate size and quality because nucleation and growth processes can be unsuccessful. Here, we show that by adsorbing proteins onto porous polystyrene-divinylbenzene microspheres (SDB) floating on the surface of the crystallisation solution, a localised high supersaturation region at the surface of the microspheres and a low supersaturation region below the microspheres can coexist in a single solution. The crystals will easily nucleate in the region of high supersaturation, but when they grow to a certain size, they will sediment to the region of low supersaturation and continue to grow. In this way, the probability of crystallisation and crystal quality can be simultaneously increased in a single solution without changing other crystallisation parameters.
KeywordDynamic Light-scattering Heterogeneous Nucleation Separating Nucleation Structural Genomics Lysozyme Surfaces Optimization Orientation Nucleants Growth
Indexed BySCI
Language英语
WOS IDWOS:000346267700002
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/14548
Collection中科院上海应用物理研究所2011-2020年
Corresponding AuthorChristian.Betzel@uni-hamburg.de; hejianhua@sinap.ac.cn; yindc@nwpu.edu.cn
Recommended Citation
GB/T 7714
Guo, YZ,Sun, LH,Oberthuer, D,et al. Utilisation of adsorption and desorption for simultaneously improving protein crystallisation success rate and crystal quality[J]. SCIENTIFIC REPORTS,2014,4.
APA Guo, YZ.,Sun, LH.,Oberthuer, D.,Zhang, CY.,Shi, JY.,...&yindc@nwpu.edu.cn.(2014).Utilisation of adsorption and desorption for simultaneously improving protein crystallisation success rate and crystal quality.SCIENTIFIC REPORTS,4.
MLA Guo, YZ,et al."Utilisation of adsorption and desorption for simultaneously improving protein crystallisation success rate and crystal quality".SCIENTIFIC REPORTS 4(2014).
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