Knowledge Management System Of Shanghai Institute of Applied Physics, CAS
| Utilisation of adsorption and desorption for simultaneously improving protein crystallisation success rate and crystal quality | |
| Guo, YZ; Sun, LH; Oberthuer, D; Zhang, CY; Shi, JY; Di, JL; Zhang, BL; Cao, HL; Liu, YM; Li, J; Wang, Q; Huang, HH; Liu, J; Schulz, JM; Zhang, QY; Zhao, JL; Betzel, C; He, JH; Yin, DC; Christian.Betzel@uni-hamburg.de; hejianhua@sinap.ac.cn; yindc@nwpu.edu.cn | |
| 2014 | |
| Source Publication | SCIENTIFIC REPORTS
 |
| ISSN | 2045-2322 |
| Volume | 4 |
| Abstract | High-quality protein crystals of suitable size are an important prerequisite for applying X-ray crystallography to determine the 3-dimensional structure of proteins. However, it is often difficult to obtain protein crystals of appropriate size and quality because nucleation and growth processes can be unsuccessful. Here, we show that by adsorbing proteins onto porous polystyrene-divinylbenzene microspheres (SDB) floating on the surface of the crystallisation solution, a localised high supersaturation region at the surface of the microspheres and a low supersaturation region below the microspheres can coexist in a single solution. The crystals will easily nucleate in the region of high supersaturation, but when they grow to a certain size, they will sediment to the region of low supersaturation and continue to grow. In this way, the probability of crystallisation and crystal quality can be simultaneously increased in a single solution without changing other crystallisation parameters. |
| Keyword | Dynamic Light-scattering Heterogeneous Nucleation Separating Nucleation Structural Genomics Lysozyme Surfaces Optimization Orientation Nucleants Growth |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000346267700002 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/14548 |
| Collection | 中科院上海应用物理研究所2011-2020年 |
| Corresponding Author | Christian.Betzel@uni-hamburg.de; hejianhua@sinap.ac.cn; yindc@nwpu.edu.cn |
| Recommended Citation GB/T 7714 | Guo, YZ,Sun, LH,Oberthuer, D,et al. Utilisation of adsorption and desorption for simultaneously improving protein crystallisation success rate and crystal quality[J]. SCIENTIFIC REPORTS,2014,4. |
| APA | Guo, YZ.,Sun, LH.,Oberthuer, D.,Zhang, CY.,Shi, JY.,...&yindc@nwpu.edu.cn.(2014).Utilisation of adsorption and desorption for simultaneously improving protein crystallisation success rate and crystal quality.SCIENTIFIC REPORTS,4. |
| MLA | Guo, YZ,et al."Utilisation of adsorption and desorption for simultaneously improving protein crystallisation success rate and crystal quality".SCIENTIFIC REPORTS 4(2014). |
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| Utilisation of adsor（2657KB） | 开放获取 | CC BY-NC-SA | View Download | |||
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