CAS OpenIR  > 中科院上海应用物理研究所2011-2019年
A highly sensitive chemiluminescence sensor for detecting mercury (II) ions: a combination of Exonuclease III-aided signal amplification and graphene oxide-assisted background reduction
Tian, Y; Wang, Y; Xu, Y; Liu, Y; Li, D; Fan, CH
2015
Source PublicationSCIENCE CHINA-CHEMISTRY
Volume58Issue:3Pages:514—518
Subtype期刊文献
AbstractIn this paper, we report a highly sensitive chemiluminescence (CL) sensor for Hg2+ ions based on thymine-Hg2+-thymine (T-Hg2+-T) coordination chemistry. We designed a thymine rich oligonucleotide as a capture probe and a signal probe that includes two functional domains: a horseradish peroxidase-mimicking DNAzyme domain for the generation of CL, and a recognition domain. Graphene oxide (GO) was introduced to adsorb the signal probe via p-p interaction, which brought the DNAzyme domain and GO into close proximity and quenches CL. In the presence of Hg2+ ions, the coordination of Hg2+ with the capture probe yielded a hairpin complex, triggers cascaded strand displacement reactions and Exonuclease III-assisted signal amplifications. As a result, accumulated amounts of DNAzyme were generated and released from GO, leading to an enhanced CL signal. This strategy combines enzyme-based signal amplification and GO as a background reducer, leads to a limit of detection (LOD) of 2 nmol/L. This simple detection system provides a label-free yet sensitive approach for detection of Hg2+ ions.
KeywordCdse/zns Quantum Dots Gold Nanoparticles Dna Detection Metal-ions Label-free Hg2++ Ions Dnazyme Duplexes Water Cu2++
Indexed BySCI
Language英语
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/24370
Collection中科院上海应用物理研究所2011-2019年
Recommended Citation
GB/T 7714
Tian, Y,Wang, Y,Xu, Y,et al. A highly sensitive chemiluminescence sensor for detecting mercury (II) ions: a combination of Exonuclease III-aided signal amplification and graphene oxide-assisted background reduction[J]. SCIENCE CHINA-CHEMISTRY,2015,58(3):514—518.
APA Tian, Y,Wang, Y,Xu, Y,Liu, Y,Li, D,&Fan, CH.(2015).A highly sensitive chemiluminescence sensor for detecting mercury (II) ions: a combination of Exonuclease III-aided signal amplification and graphene oxide-assisted background reduction.SCIENCE CHINA-CHEMISTRY,58(3),514—518.
MLA Tian, Y,et al."A highly sensitive chemiluminescence sensor for detecting mercury (II) ions: a combination of Exonuclease III-aided signal amplification and graphene oxide-assisted background reduction".SCIENCE CHINA-CHEMISTRY 58.3(2015):514—518.
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