CAS OpenIR  > 中科院上海应用物理研究所2011-2019年
A novel nanobody specific for respiratory surfactant protein A has potential for lung targeting
Wang, SM; He, X; Li, N; Yu, F; Hu, Y; Wang, LS; Zhang, P; Du, YK; Du, SS; Yin, ZF; Wei, YR; Mulet, X; Coia, G; Weng, D; He, JH; Wu, M; Li, HP
2015
Source PublicationINTERNATIONAL JOURNAL OF NANOMEDICINE
Volume10Pages:2857—2869
Subtype期刊文献
AbstractLung-targeting drugs are thought to be potential therapies of refractory lung diseases by maximizing local drug concentrations in the lung to avoid systemic circulation. However, a major limitation in developing lung-targeted drugs is the acquirement of lung-specific ligands. Pulmonary surfactant protein A (SPA) is predominantly synthesized by type II alveolar epithelial cells, and may serve as a potential lung-targeting ligand. Here, we generated recombinant rat pulmonary SPA (rSPA) as an antigen and immunized an alpaca to produce two nanobodies (the smallest naturally occurring antibodies) specific for rSPA, designated Nb6 and Nb17. To assess these nanobodies' potential for lung targeting, we evaluated their specificity to lung tissue and toxicity in mice. Using immunohistochemistry, we demonstrated that these anti-rSPA nanobodies selectively bound to rat lungs with high affinity. Furthermore, we intravenously injected fluorescein isothiocyanate-Nb17 in nude mice and observed its preferential accumulation in the lung to other tissues, suggesting high affinity of the nanobody for the lung. Studying acute and chronic toxicity of Nb17 revealed its safety in rats without causing apparent histological alterations. Collectively, we have generated and characterized lung-specific nanobodies, which may be applicable for lung drug delivery.
KeywordInhaled Corticosteroids Antibody Fragments Albumin Nanoparticles Cancer-cells Tumor-cells Delivery Therapy Growth Inhibition Expression
Indexed BySCI
Language英语
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/24380
Collection中科院上海应用物理研究所2011-2019年
Recommended Citation
GB/T 7714
Wang, SM,He, X,Li, N,et al. A novel nanobody specific for respiratory surfactant protein A has potential for lung targeting[J]. INTERNATIONAL JOURNAL OF NANOMEDICINE,2015,10:2857—2869.
APA Wang, SM.,He, X.,Li, N.,Yu, F.,Hu, Y.,...&Li, HP.(2015).A novel nanobody specific for respiratory surfactant protein A has potential for lung targeting.INTERNATIONAL JOURNAL OF NANOMEDICINE,10,2857—2869.
MLA Wang, SM,et al."A novel nanobody specific for respiratory surfactant protein A has potential for lung targeting".INTERNATIONAL JOURNAL OF NANOMEDICINE 10(2015):2857—2869.
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