CAS OpenIR  > 中科院上海应用物理研究所2011-2019年
A novel ultrasensitive electrochemical DNA sensor based on double tetrahedral nanostructures
Zeng, DD; Zhang, H; Zhu, D; Li, J; San, LL; Wang, ZH; Wang, CG; Wang, YS; Wang, LH; Zuo, XL; Mi, XQ
2015
Source PublicationBIOSENSORS & BIOELECTRONICS
Volume71Pages:434—438
Subtype期刊文献
AbstractElectrochemical DNA (E-DNA) sensor is an important tool for detecting DNA biomarker. In this work, we have demonstrated a novel strategy of E-DNA sensor based on DNA tetrahedral nanostructures for the sensitive detection of target DNA. In our design, thiol and biotin modified DNA tetrahedral nanostructures were used as capture and report probes respectively. The biotin-tagged three dimensional DNA tetrahedral nanostructures were employed for efficient signal amplification by capturing multiple catalytic enzymes. Such improved E-DNA sensor can sensitively detect DNA target as low as 1 fM with excellent differentiation ability for even single mismatch. And a mean recovery rate of 90.57% in DNA solution extracted from human serum was obtained. We have also compared this new method of attaching catalytic enzymes with the other two typical methods: One is through biotinylated single-stranded DNA (SSDNA) and the other is through gold nanoparticles (GNPs). Results indicated that the RTSPs-based enzyme amplification system showed much better performance than the other two systems. (C) 2015 Elsevier B.V. All rights reserved.
KeywordSequence-specific Detection Amplification Hybridization Biosensor
Indexed BySCI
Language英语
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/24381
Collection中科院上海应用物理研究所2011-2019年
Recommended Citation
GB/T 7714
Zeng, DD,Zhang, H,Zhu, D,et al. A novel ultrasensitive electrochemical DNA sensor based on double tetrahedral nanostructures[J]. BIOSENSORS & BIOELECTRONICS,2015,71:434—438.
APA Zeng, DD.,Zhang, H.,Zhu, D.,Li, J.,San, LL.,...&Mi, XQ.(2015).A novel ultrasensitive electrochemical DNA sensor based on double tetrahedral nanostructures.BIOSENSORS & BIOELECTRONICS,71,434—438.
MLA Zeng, DD,et al."A novel ultrasensitive electrochemical DNA sensor based on double tetrahedral nanostructures".BIOSENSORS & BIOELECTRONICS 71(2015):434—438.
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