CAS OpenIR  > 中科院上海应用物理研究所2011-2019年
Binding-induced collapse of DNA nano-assembly for naked-eye detection of ATP with plasmonic gold nanoparticles
Wang, JJ; Lu, JX; Su, S; Gao, JM; Huang, Q; Wang, LH; Huang, W; Zuo, XL
2015
Source PublicationBIOSENSORS & BIOELECTRONICS
Volume65Pages:171—175
Subtype期刊文献
AbstractThe detection of small molecules depends heavily on complicated GC-MS (Gas chromatography-mass spectrometry), HPLC (High-performance liquid chromatography) and some other complicated instruments that are not suitable for point of care detection. Here, we have demonstrated a fast (in 10 min), simple (instrument-free) and effective detection platform for small molecule-ATP. In our design, we engineered the hybridization region of aptamer and assembled it into a superstructure to avoid the exposed flexible ends. The binding of ATP triggered the collapse of the superstructures to produce single stranded DNA that can obviously tune the plasmonic coupling of unmodified gold nanoparticles (AuNPs). Compared to detection platforms based on fully hybridized aptamer double helix, the detection time was significantly decreased to 10 min. The resulting color change can be recognized by naked eyes. Our detection is highly specific and selective. Furthermore, a logic gate with multiplexed detection capability for ATP and DNA were demonstrated. (C) 2014 Elsevier B.V. All rights reserved.
KeywordSmall Molecules Colorimetric Detection Aptamer Aptasensor Signal Probe Assay Amplification Diagnostics Sequences
Indexed BySCI
Language英语
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/24400
Collection中科院上海应用物理研究所2011-2019年
Recommended Citation
GB/T 7714
Wang, JJ,Lu, JX,Su, S,et al. Binding-induced collapse of DNA nano-assembly for naked-eye detection of ATP with plasmonic gold nanoparticles[J]. BIOSENSORS & BIOELECTRONICS,2015,65:171—175.
APA Wang, JJ.,Lu, JX.,Su, S.,Gao, JM.,Huang, Q.,...&Zuo, XL.(2015).Binding-induced collapse of DNA nano-assembly for naked-eye detection of ATP with plasmonic gold nanoparticles.BIOSENSORS & BIOELECTRONICS,65,171—175.
MLA Wang, JJ,et al."Binding-induced collapse of DNA nano-assembly for naked-eye detection of ATP with plasmonic gold nanoparticles".BIOSENSORS & BIOELECTRONICS 65(2015):171—175.
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