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Chemical repair activity of free radical scavenger edaravone: reduction reactions with dGMP hydroxyl radical adducts and suppression of base lesions and AP sites on irradiated plasmid DNA
Hata, K; Urushibara, A; Yamashita, S; Lin, MZ; Muroya, Y; Shikazono, N; Yokoya, A; Fu, HY; Katsumura, Y
2015
Source PublicationJOURNAL OF RADIATION RESEARCH
Volume56Issue:1Pages:59—66
Subtype期刊文献
AbstractReactions of edaravone (3-methyl-1-phenyl-2-pyrazolin-5-one) with deoxyguanosine monophosphate (dGMP) hydroxyl radical adducts were investigated by pulse radiolysis technique. Edaravone was found to reduce the dGMP hydroxyl radical adducts through electron transfer reactions. The rate constants of the reactions were greater than 4 x 10(8) dm(3) mol(-1) s(-1) and similar to those of the reactions of ascorbic acid, which is a representative antioxidant. Yields of single-strand breaks, base lesions, and abasic sites produced in pUC18 plasmid DNA by gamma ray irradiation in the presence of low concentrations (10-1000 mu mol dm(-3)) of edaravone were also quantified, and the chemical repair activity of edaravone was estimated by a method recently developed by the authors. By comparing suppression efficiencies to the induction of each DNA lesion, it was found that base lesions and abasic sites were suppressed by the chemical repair activity of edaravone, although the suppression of single-strand breaks was not very effective. This phenomenon was attributed to the chemical repair activity of edaravone toward base lesions and abasic sites. However, the chemical repair activity of edaravone for base lesions was lower than that of ascorbic acid.
KeywordTransient Focal Ischemia Induced Single-strand Pulse-radiolysis Peroxyl Radicals Antioxidant Activity Reperfusion Injury Cerebral-ischemia Aqueous-solution Mammalian-cells Ascorbic-acid
Indexed BySCI
Language英语
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/24412
Collection中科院上海应用物理研究所2011-2019年
Recommended Citation
GB/T 7714
Hata, K,Urushibara, A,Yamashita, S,et al. Chemical repair activity of free radical scavenger edaravone: reduction reactions with dGMP hydroxyl radical adducts and suppression of base lesions and AP sites on irradiated plasmid DNA[J]. JOURNAL OF RADIATION RESEARCH,2015,56(1):59—66.
APA Hata, K.,Urushibara, A.,Yamashita, S.,Lin, MZ.,Muroya, Y.,...&Katsumura, Y.(2015).Chemical repair activity of free radical scavenger edaravone: reduction reactions with dGMP hydroxyl radical adducts and suppression of base lesions and AP sites on irradiated plasmid DNA.JOURNAL OF RADIATION RESEARCH,56(1),59—66.
MLA Hata, K,et al."Chemical repair activity of free radical scavenger edaravone: reduction reactions with dGMP hydroxyl radical adducts and suppression of base lesions and AP sites on irradiated plasmid DNA".JOURNAL OF RADIATION RESEARCH 56.1(2015):59—66.
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