CAS OpenIR  > 中科院上海应用物理研究所2011-2018年
Electrochemical DNA Biosensor Based on a Tetrahedral Nanostructure Probe for the Detection of Avian Influenza A (H7N9) Virus
Dong, SB; Zhao, RT; Zhu, JG; Lu, X; Li, Y; Qiu, SF; Jia, LL; Jiao, X; Song, SP; Fan, CH; Hao, RZ; Song, HB
2015
Source PublicationACS APPLIED MATERIALS & INTERFACES
Volume7Issue:16Pages:8834—8842
Subtype期刊文献
AbstractA DNA tetrahedral nanostructure-based electrochemical biosensor was developed to detect avian influenza A (H7N9) virus through recognizing a fragment of the hemagglutinin gene sequence. The DNA tetrahedral probe was immobilized onto a gold electrode surface based on self-assembly between three thiolated nucleotide sequences and a longer nucleotide sequence containing complementary DNA to hybridize with the target single-stranded (ss)DNA. The captured target sequence was hybridized with a biotinylated-ssDNA oligonucleotide as a detection probe, and then avidin-horseradish peroxidase was introduced to produce an amperometric signal through the interaction with 3,3',5,5'-tetramethylbenzidine substrate. The target ssDNA was obtained by asymmetric polymerase chain reaction (PCR) of the cDNA template, reversely transcribed from the viral lysate of influenza A (H7N9) virus in throat swabs. The results showed that this electrochemical biosensor could specifically recognize the target DNA fragment of influenza A (H7N9) virus from other types of influenza viruses, such as influenza A (H1N1) and (H3N2) viruses, and even from single-base mismatches of oligonucleotides. Its detection limit could reach a magnitude of 100 fM for target nucleotide sequences. Moreover, the cycle number of the asymmetric PCR could be reduced below three with the electrochemical biosensor still distinguishing the target sequence from the negative control. To the best of our knowledge, this is the first report of the detection of target DNA from clinical samples using a tetrahedral DNA probe functionalized electrochemical biosensor. It displays that the DNA tetrahedra has a great potential application as a probe of the electrochemical biosensor to detect avian influenza A (H7N9) virus and other pathogens at the gene level, which will potentially aid the prevention and control of the disease caused by such pathogens.
KeywordGold Electrodes Label-free Hybridization Sequence Platform Sensor Gene Lna Immobilization Amplification
Indexed BySCI
Language英语
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/24450
Collection中科院上海应用物理研究所2011-2018年
Recommended Citation
GB/T 7714
Dong, SB,Zhao, RT,Zhu, JG,et al. Electrochemical DNA Biosensor Based on a Tetrahedral Nanostructure Probe for the Detection of Avian Influenza A (H7N9) Virus[J]. ACS APPLIED MATERIALS & INTERFACES,2015,7(16):8834—8842.
APA Dong, SB.,Zhao, RT.,Zhu, JG.,Lu, X.,Li, Y.,...&Song, HB.(2015).Electrochemical DNA Biosensor Based on a Tetrahedral Nanostructure Probe for the Detection of Avian Influenza A (H7N9) Virus.ACS APPLIED MATERIALS & INTERFACES,7(16),8834—8842.
MLA Dong, SB,et al."Electrochemical DNA Biosensor Based on a Tetrahedral Nanostructure Probe for the Detection of Avian Influenza A (H7N9) Virus".ACS APPLIED MATERIALS & INTERFACES 7.16(2015):8834—8842.
Files in This Item: Download All
File Name/Size DocType Version Access License
Electrochemical DNA (1704KB)期刊论文作者接受稿开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Dong, SB]'s Articles
[Zhao, RT]'s Articles
[Zhu, JG]'s Articles
Baidu academic
Similar articles in Baidu academic
[Dong, SB]'s Articles
[Zhao, RT]'s Articles
[Zhu, JG]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Dong, SB]'s Articles
[Zhao, RT]'s Articles
[Zhu, JG]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Electrochemical DNA Biosensor Based on a Tetrahedral Nanostructure Probe for the Detection of Avian Influenza A (H7N9) Virus.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.