CAS OpenIR  > 中科院上海应用物理研究所2011-2019年
Expression, crystallization and preliminary crystallographic data analysis of VioD, a hydroxylase in the violacein-biosynthesis pathway
Ran, TT; Gao, MX; Wei, QE; He, JH; Tang, L; Wang, WW; Xu, DQ
2015
Source PublicationACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
Volume71Pages:149—152
Subtype期刊文献
AbstractViolacein, a natural purple secondary metabolite, is sequentially biosynthesized by five enzymes in the following pathway: VioA-VioB-VioE-VioD-VioC. VioD, a flavin-dependent oxygenase, catalyzes the hydroxylation of the intermediate product prodeoxyviolaceinic acid (PVA) at the 5-position of one indole ring to yield proviolacein. In vitro biochemical data have revealed this process, but the catalytic mechanism still remains largely unclear. Here, the cloning, expression, purification, crystallization and diffraction of VioD are reported. Crystals of VioD diffracted to 1.7 angstrom resolution and belonged to space group P3(1), with unit-cell parameters a = b = 90.0, c = 94.5 angstrom, alpha = beta = 90, gamma = 120 degrees. Solvent-content calculation and molecular-replacement results suggest the presence of two molecules of VioD in the asymmetric unit.
KeywordChromobacterium-violaceum Janthinobacterium-lividum Heterologous Expression Gene-cluster Rearrangement Trypanocide Tryptophan Molecule Cloning
Indexed BySCI
Language英语
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/24459
Collection中科院上海应用物理研究所2011-2019年
Recommended Citation
GB/T 7714
Ran, TT,Gao, MX,Wei, QE,et al. Expression, crystallization and preliminary crystallographic data analysis of VioD, a hydroxylase in the violacein-biosynthesis pathway[J]. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS,2015,71:149—152.
APA Ran, TT.,Gao, MX.,Wei, QE.,He, JH.,Tang, L.,...&Xu, DQ.(2015).Expression, crystallization and preliminary crystallographic data analysis of VioD, a hydroxylase in the violacein-biosynthesis pathway.ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS,71,149—152.
MLA Ran, TT,et al."Expression, crystallization and preliminary crystallographic data analysis of VioD, a hydroxylase in the violacein-biosynthesis pathway".ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS 71(2015):149—152.
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