CAS OpenIR  > 中科院上海应用物理研究所2011-2019年
Fluorescent In Situ Targeting Probes for Rapid Imaging of Ovarian-Cancer-Specific gamma-Glutamyltranspeptidase
Wang, FY; Zhu, Y; Zhou, L; Pan, L; Cui, ZF; Fei, Q; Luo, SH; Pan, D; Huang, Q; Wang, R; Zhao, CC; Tian, H; Fan, CH
2015
Source PublicationANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume54Issue:25Pages:7349—7353
Subtype期刊文献
Abstractgamma-Glutamyltranspeptidase (GGT) is a tumor biomarker that selectively catalyzes the cleavage of glutamate overexpressed on the plasma membrane of tumor cells. Here, we developed two novel fluorescent in situ targeting (FIST) probes that specifically target GGT in tumor cells, which comprise 1) a GGT-specific substrate unit (GSH), and 2) a boron-dipyrromethene (BODIPY) moiety for fluorescent signalling. In the presence of GGT, sulfur-substituted BODIPY was converted to amino-substituted BODIPY, resulting in dramatic fluorescence variations. By exploiting this enzyme-triggered photophysical property, we employed these FIST probes to monitor the GGT activity in living cells, which showed remarkable differentiation between ovarian cancer cells and normal cells. These probes represent two first-generation chemodosimeters featuring enzyme-mediated rapid, irreversible aromatic hydrocarbon transfer between the sulfur and nitrogen atoms accompanied by switching of photophysical properties.
KeywordProtease Activity Fluorogenic Probe Turn-on Cells Design Glutathione Cysteine Transferase Glutamyltransferase Homocysteine
Indexed BySCI
Language英语
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/24460
Collection中科院上海应用物理研究所2011-2019年
Recommended Citation
GB/T 7714
Wang, FY,Zhu, Y,Zhou, L,et al. Fluorescent In Situ Targeting Probes for Rapid Imaging of Ovarian-Cancer-Specific gamma-Glutamyltranspeptidase[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2015,54(25):7349—7353.
APA Wang, FY.,Zhu, Y.,Zhou, L.,Pan, L.,Cui, ZF.,...&Fan, CH.(2015).Fluorescent In Situ Targeting Probes for Rapid Imaging of Ovarian-Cancer-Specific gamma-Glutamyltranspeptidase.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,54(25),7349—7353.
MLA Wang, FY,et al."Fluorescent In Situ Targeting Probes for Rapid Imaging of Ovarian-Cancer-Specific gamma-Glutamyltranspeptidase".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 54.25(2015):7349—7353.
Files in This Item: Download All
File Name/Size DocType Version Access License
Fluorescent In Situ (1321KB)期刊论文作者接受稿开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wang, FY]'s Articles
[Zhu, Y]'s Articles
[Zhou, L]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wang, FY]'s Articles
[Zhu, Y]'s Articles
[Zhou, L]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wang, FY]'s Articles
[Zhu, Y]'s Articles
[Zhou, L]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Fluorescent In Situ Targeting Probes for Rapid Imaging of Ovarian-Cancer-Specific gamma-Glutamyltranspeptidase.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.