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Improved resolution in fluorescence microscopy with the FRET pairs by time gating | |
Hou, SG; Chen, JF; Deng, SH; Wang, F; Huang, Q; Cheng, Y; Fan, CH | |
2015 | |
Source Publication | OPTICS EXPRESS
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Volume | 23Issue:10Pages:13121—13129 |
Subtype | 期刊文献 |
Abstract | We investigate the temporal information of nonlinear donor fluorescence in the FRET frustration microscopy. The arrival time of the donor fluorescence varies with the distribution of the excitation laser. The differences of the arrival times between different positions in the excitation spot can be further enhanced in the case of adding a depletion beam upon the FRET probes. The spatial information is encoded in the temporal dynamics of the fluorescent photons from donor molecules and time-gating in detection can be used to increase the spatial resolution. (C) 2015 Optical Society of America |
Keyword | Resonance Energy-transfer Nanoscopy 2-photon |
Indexed By | SCI |
Language | 英语 |
Document Type | 期刊论文 |
Identifier | http://ir.sinap.ac.cn/handle/331007/24474 |
Collection | 中科院上海应用物理研究所2011-2020年 |
Recommended Citation GB/T 7714 | Hou, SG,Chen, JF,Deng, SH,et al. Improved resolution in fluorescence microscopy with the FRET pairs by time gating[J]. OPTICS EXPRESS,2015,23(10):13121—13129. |
APA | Hou, SG.,Chen, JF.,Deng, SH.,Wang, F.,Huang, Q.,...&Fan, CH.(2015).Improved resolution in fluorescence microscopy with the FRET pairs by time gating.OPTICS EXPRESS,23(10),13121—13129. |
MLA | Hou, SG,et al."Improved resolution in fluorescence microscopy with the FRET pairs by time gating".OPTICS EXPRESS 23.10(2015):13121—13129. |
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Improved resolution (1241KB) | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Download |
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