CAS OpenIR  > 中科院上海应用物理研究所2011-2020年
Nanodiamonds act as Trojan horse for intracellular delivery of metal ions to trigger cytotoxicity
Zhu, Y; Zhang, Y; Shi, GS; Yang, JR; Zhang, JC; Li, WX; Li, AG; Tai, RZ; Fang, HP; Fan, CH; Huang, Q
AbstractBackground: Nanomaterials hold great promise for applications in the delivery of various molecules with poor cell penetration, yet its potential for delivery of metal ions is rarely considered. Particularly, there is limited insight about the cytotoxicity triggered by nanoparticle-ion interactions. Oxidative stress is one of the major toxicological mechanisms for nanomaterials, and we propose that it may also contribute to nanoparticle-ion complexes induced cytotoxicity. Methods: To explore the potential of nanodiamonds (NDs) as vehicles for metal ion delivery, we used a broad range of experimental techniques that aimed at getting a comprehensive assessment of cell responses after exposure of NDs, metal ions, or ND-ion mixture: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, Trypan blue exclusion text, optical microscope observation, synchrotron-based scanning transmission X-ray microscopy (STXM) and micro X-ray fluorescence (mu XRF) microscopy, inductively coupled plasma-mass spectrometry (ICP-MS), reactive oxygen species (ROS) assay and transmission electron microscopy (TEM) observation. In addition, theoretical calculation and molecular dynamics (MD) computation were used to illustrate the adsorption properties of different metal ion on NDs as well as release profile of ion from ND-ion complexes at different pH values. Results: The adsorption capacity of NDs for different metal ions was different, and the adsorption for Cu2+ was the most strong among divalent metal ions. These different ND-ion complexes then had different cytotoxicity by influencing the subsequent cellular responses. Detailed investigation of ND-Cu2+ interaction showed that the amount of released Cu2+ from ND-Cu2+ complexes at acidic lysosomal conditions was much higher than that at neutral conditions, leading to the elevation of intracellular ROS level, which triggered cytotoxicity. By theoretical approaches, we demonstrated that the functional carbon surface and cluster structures of NDs made them good vehicles for metal ions delivery. Conclusions: NDs played the Trojan horse role by allowing large amounts of metal ions accumulate into living cells followed by subsequent release of ions in the interior of cells, which then led to cytotoxicity. The present experimental and theoretical results provide useful insight into understanding of cytotoxicity triggered by nanoparticle-ion interactions, and open new ways in the interpretation of nanotoxicity.
KeywordCarbon Nanotubes Sirna Delivery Drug-delivery Cells Nanoparticles Transporters Copper Model Nanomaterials Geometries
Indexed BySCI
Document Type期刊论文
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GB/T 7714
Zhu, Y,Zhang, Y,Shi, GS,et al. Nanodiamonds act as Trojan horse for intracellular delivery of metal ions to trigger cytotoxicity[J]. PARTICLE AND FIBRE TOXICOLOGY,2015,12:—.
APA Zhu, Y.,Zhang, Y.,Shi, GS.,Yang, JR.,Zhang, JC.,...&Huang, Q.(2015).Nanodiamonds act as Trojan horse for intracellular delivery of metal ions to trigger cytotoxicity.PARTICLE AND FIBRE TOXICOLOGY,12,—.
MLA Zhu, Y,et al."Nanodiamonds act as Trojan horse for intracellular delivery of metal ions to trigger cytotoxicity".PARTICLE AND FIBRE TOXICOLOGY 12(2015):—.
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