CAS OpenIR  > 中科院上海应用物理研究所2011-2019年
Quantitative investigation of the poly-adenine DNA dissociation from the surface of gold nanoparticles
Lu, WW; Wang, LH; Li, J; Zhao, Y; Zhou, Z; Shi, JY; Zuo, XL; Pan, D
2015
Source PublicationSCIENTIFIC REPORTS
Volume5Pages:
Subtype期刊文献
AbstractIn recent years, poly adenine (polyA) DNA functionalized gold nanoparticles (AuNPs) free of modifications was fabricated with high density of DNA attachment and high hybridization ability similar to those of its thiolated counterpart. This nanoconjugate utilized poly adenine as an anchoring block for binding with the AuNPs surface thereby facilitated the appended recognition block a better upright conformation for hybridization, demonstrating its great potential to be a tunable plasmonic biosensor. It's one of the key points for any of the practical applications to maintaining stable conjugation between DNA oligonucleotides and gold nanoparticles under various experimental treatments. Thus, in this research, we designed a simple but sensitive fluorescence turn-on strategy to systematically investigate and quantified the dissociation of polyA DNA on gold nanoparticles in diverse experimental conditions. DNA desorbed spontaneously as a function of elevated temperature, ion strength, buffer pH, organic solvents and keeping time. What's more, evaluating this conjugate stability as affected by the length of its polyA anchor was another crucial aspect in our study. With the improved understanding from these results, we were able to control some of our experimental conditions to maintain a good stability of this kind of polyA DNA-AuNPs nanoconjugates.
KeywordColorimetric Detection Optical-properties Thermal-stability Nonthiolated Dna Gene-regulation Oligonucleotide Functionalization Polynucleotides Nanotechnology Hybridization
Indexed BySCI
Language英语
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/24549
Collection中科院上海应用物理研究所2011-2019年
Recommended Citation
GB/T 7714
Lu, WW,Wang, LH,Li, J,et al. Quantitative investigation of the poly-adenine DNA dissociation from the surface of gold nanoparticles[J]. SCIENTIFIC REPORTS,2015,5:—.
APA Lu, WW.,Wang, LH.,Li, J.,Zhao, Y.,Zhou, Z.,...&Pan, D.(2015).Quantitative investigation of the poly-adenine DNA dissociation from the surface of gold nanoparticles.SCIENTIFIC REPORTS,5,—.
MLA Lu, WW,et al."Quantitative investigation of the poly-adenine DNA dissociation from the surface of gold nanoparticles".SCIENTIFIC REPORTS 5(2015):—.
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