CAS OpenIR  > 中科院上海应用物理研究所2011-2019年
Rational Designed Bipolar, Conjugated Polymer-DNA Composite Beacon for the Sensitive Detection of Proteins and Ions
Jia, YM; Zuo, XL; Lou, XD; Miao, M; Cheng, Y; Min, XH; Li, XC; Xia, F
2015
Source PublicationANALYTICAL CHEMISTRY
Volume87Issue:7Pages:3890—3894
Subtype期刊文献
AbstractNature owns remarkable capabilities in sensing target molecules, while the artificial biosensor lags far behind nature. Inspired by nature, we devise a new sensing platform that can specifically bind the molecules and synchronously initiate a specific signal response. We rationally designed a type of bipolar probe that is comprised of a hydrophilic DNA part and a hydrophobic conjugated polymer (CP) unit. In aqueous solution, they can form micelles with a hydrophobic CP core and a hydrophilic DNA shell. The aggregation-caused quenching suppresses the fluorescence of CP. Adding telomerase, the hydropathical profile of the bipolar probes is drastically regulated that results in the collapse of micelles and liberates fluorescence simultaneously. The probe has been used in both mimic systems and real urine samples (38 samples). We achieve sensitive and specific detection of telomerase and obtain clearly classification for normal people and cancer patients. It can also be used in a signal off sensor that is used to detect mercury ions.
KeywordEnhanced Raman-scattering Human Telomerase Activity Molecular Beacons Electrochemical Biosensor Ultrasensitive Detection Carbon Nanotubes In-vitro Aptamer Cells Hybridization
Indexed BySCI
Language英语
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/24551
Collection中科院上海应用物理研究所2011-2019年
Recommended Citation
GB/T 7714
Jia, YM,Zuo, XL,Lou, XD,et al. Rational Designed Bipolar, Conjugated Polymer-DNA Composite Beacon for the Sensitive Detection of Proteins and Ions[J]. ANALYTICAL CHEMISTRY,2015,87(7):3890—3894.
APA Jia, YM.,Zuo, XL.,Lou, XD.,Miao, M.,Cheng, Y.,...&Xia, F.(2015).Rational Designed Bipolar, Conjugated Polymer-DNA Composite Beacon for the Sensitive Detection of Proteins and Ions.ANALYTICAL CHEMISTRY,87(7),3890—3894.
MLA Jia, YM,et al."Rational Designed Bipolar, Conjugated Polymer-DNA Composite Beacon for the Sensitive Detection of Proteins and Ions".ANALYTICAL CHEMISTRY 87.7(2015):3890—3894.
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