CAS OpenIR  > 中科院上海应用物理研究所2011-2019年
Unraveling the Role of Hydrogen Peroxide in alpha-Synuclein Aggregation Using an Ultrasensitive Nanoplasmonic Probe
Xu, Y; Li, K; Qin, WW; Zhu, B; Zhou, Z; Shi, JY; Wang, K; Hu, J; Fan, CH; Li, D
2015
Source PublicationANALYTICAL CHEMISTRY
Volume87Issue:3Pages:1968—1973
Subtype期刊文献
AbstractAggregation of alpha-Synuclein (alpha-Syn) in Lewy bodies is largely responsible for the demise and death of dopamine neurons. Oxidative stress associated with the aggregation-induced oxidative damage is considered as a possible origin of the toxicity. However, the cellular mechanism of H2O2 in the aggregation of alpha-Syn remains a debate, i.e., whether the aggregation is caused by endogenously secreted or exogenous H2O2 from upstream. Here, we report on the development of an ultrasensitive plasmonic assay with a designed nanoplasmonic probe to unravel the role of H2O2 in the aggregation of alpha-Syn. The nanoplasmonic probe is composed of a Au nanoparticle with surface-attached double-stranded DNA and horseradish peroxidase (HRP). In the presence of H2O2, HRP initiates the polymerization of aniline, which in turn results in the in situ formation of a layer of conducting polymer on the nanoplasmonic probe. By monitoring the associated plasmonic response, we can sensitively detect H2O2 with a remarkably low detection limit of 8 nM. With this ultrasensitive plasmonic assay, we find that exogenous H2O2 plays a dominant role for the aggregation of alpha-Syn in vitro, whereas the contribution from endogenously secreted H2O2 is negligible.
KeywordParkinsons-disease Gold Nanoparticles Au Nanoparticles Cell-death In-vitro H2o2 Polyaniline Biosensors Hybridization Pathways
Indexed BySCI
Language英语
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/24620
Collection中科院上海应用物理研究所2011-2019年
Recommended Citation
GB/T 7714
Xu, Y,Li, K,Qin, WW,et al. Unraveling the Role of Hydrogen Peroxide in alpha-Synuclein Aggregation Using an Ultrasensitive Nanoplasmonic Probe[J]. ANALYTICAL CHEMISTRY,2015,87(3):1968—1973.
APA Xu, Y.,Li, K.,Qin, WW.,Zhu, B.,Zhou, Z.,...&Li, D.(2015).Unraveling the Role of Hydrogen Peroxide in alpha-Synuclein Aggregation Using an Ultrasensitive Nanoplasmonic Probe.ANALYTICAL CHEMISTRY,87(3),1968—1973.
MLA Xu, Y,et al."Unraveling the Role of Hydrogen Peroxide in alpha-Synuclein Aggregation Using an Ultrasensitive Nanoplasmonic Probe".ANALYTICAL CHEMISTRY 87.3(2015):1968—1973.
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