CAS OpenIR  > 中科院上海应用物理研究所2011-2019年
Constructing Higher-Order DNA Nanoarchitectures with Highly Purified DNA Nanocages
Xing, S; Jiang, DW; Li, F; Li, J; Li, Q; Huang, Q; Guo, LJ; Xia, JY; Shi, JY; Fan, CH; Zhang, L; Wang, LH; Zhang, L (reprint author), Chinese Acad Sci, Div Phys Biol & Bioimaging Ctr, Shanghai Synchrotron Radiat Facil, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China.
2015
Source PublicationACS APPLIED MATERIALS & INTERFACES
ISSN1944-8244
Volume7Issue:24Pages:13174—13179
Subtype期刊文献
AbstractDNA nanostructures have attracted great attention due to their precisely controllable geometry and great potential in various areas including bottom-up self-assembly. However, construction of higher-order DNA. nanoarchitectures with individual DNA nanostructures is often hampered with the purity and quantity of these "bricks". Here, we introduced size exclusion chromatography (SEC) to prepare highly purified :tetrahedral DNA nanocages in large scale and demonstrated that precise quantification of DNA nanocages was the key to the formation of higher-order DNA nanoarchitectures: We successfully purified a:series of DNA nanocages with different sizes, including Seven DNA tetrahedra with different edge lengths (7, 10, 13, 17, 20, 26, 30 bp) and one trigonal bipyramid with a 20-bp edge. These highly purified and aggregation-free DNA nanocages could be self-assembled into higher-order DNA nanoarchitectures with extraordinarily high yields (98% for dimer and 95% for trimer). As a comparison, unpurified DNA nanocages resulted in low yield of 14% for dimer and 12% for trimer, respectively. AFM images cleraly presented the characteristic structure of Monomer, did-net and trimer, impling the purified DNA nanocages well-formed the designed nanoarchitectures. Therefore, we have demonstrated that highly purified DNA nanocages are excellent "bricks" for DNA nanotechnology and show great potential in various applications of DNA nanomaterials.
KeywordDrug-delivery Origami Nanostructures Nanoparticles Nanotechnology Tetrahedron Scaffolds Microrna Shapes
Indexed BySCI
Language英语
WOS IDWOS:000357063200009
Citation statistics
Cited Times:22[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/24887
Collection中科院上海应用物理研究所2011-2019年
Corresponding AuthorZhang, L (reprint author), Chinese Acad Sci, Div Phys Biol & Bioimaging Ctr, Shanghai Synchrotron Radiat Facil, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China.
Recommended Citation
GB/T 7714
Xing, S,Jiang, DW,Li, F,et al. Constructing Higher-Order DNA Nanoarchitectures with Highly Purified DNA Nanocages[J]. ACS APPLIED MATERIALS & INTERFACES,2015,7(24):13174—13179.
APA Xing, S.,Jiang, DW.,Li, F.,Li, J.,Li, Q.,...&Zhang, L .(2015).Constructing Higher-Order DNA Nanoarchitectures with Highly Purified DNA Nanocages.ACS APPLIED MATERIALS & INTERFACES,7(24),13174—13179.
MLA Xing, S,et al."Constructing Higher-Order DNA Nanoarchitectures with Highly Purified DNA Nanocages".ACS APPLIED MATERIALS & INTERFACES 7.24(2015):13174—13179.
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