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Differential expression of extracellular thiol groups of moderately thermophilic Sulfobacillus thermosulfidooxidans and extremely thermophilic Acidianus manzaensis grown on S-0 and Fe2+
Liu, HC; Xia, JL; Nie, ZY; Zhen, XJ; Zhang, LJ; Xia, JL (reprint author), Cent S Univ, Sch Minerals Proc & Bioengn, Changsha 410083, Peoples R China.
2015
Source PublicationARCHIVES OF MICROBIOLOGY
ISSN0302-8933
Volume197Issue:6Pages:823—831
Subtype期刊文献
AbstractBio-oxidation of elemental sulfur (S-0) is very important in bioleaching and sulfur cycle. S-0 was proposed to be first activated by reacting with reactive thiol groups (-SH) of outer membrane proteins, forming -S (n) H (n a parts per thousand yen 2) complexes. The differential expression of -SH of moderately thermophilic Sulfobacillus thermosulfidooxidans and extremely thermophilic Acidianus manzaensis grown on Fe2+ and S-0 was investigated by synchrotron radiation-based scanning transmission X-ray microscopy (STXM) imaging and micro-beam X-ray fluorescence (mu-XRF) mapping. The STXM imaging and mu-XRF mapping of extracellular -SH were based on the analysis of Ca2+ bound on the cell. By comparing Ca2+ of the cells with and without labeling by Ca2+, the distribution and content of thiol groups were obtained. The results showed that, for both S. thermosulfidooxidans and A. manzaensis, the expression of extracellular -SH of S-0-grown cells was higher than that of Fe2+-grown cells. Statistical analysis indicated that the expression of extracellular -SH for S. thermosulfidooxidans and A. manzaensis grown on S-0 was 2.37 times and 2.14 times, respectively, to that on Fe2+. These results evidently demonstrate that the extracellular thiol groups are most probably involved in elemental sulfur activation and oxidation of the acidophilic sulfur-oxidizing microorganisms.
KeywordBacterium Allochromatium-vinosum Acidithiobacillus-ferrooxidans Sulfur-compounds Elemental Sulfur Oxidation Proteins Transformation Chalcopyrite Microscope Metabolism
Indexed BySCI
Language英语
WOS IDWOS:000358086200011
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/24899
Collection中科院上海应用物理研究所2011-2020年
Corresponding AuthorXia, JL (reprint author), Cent S Univ, Sch Minerals Proc & Bioengn, Changsha 410083, Peoples R China.
Recommended Citation
GB/T 7714
Liu, HC,Xia, JL,Nie, ZY,et al. Differential expression of extracellular thiol groups of moderately thermophilic Sulfobacillus thermosulfidooxidans and extremely thermophilic Acidianus manzaensis grown on S-0 and Fe2+[J]. ARCHIVES OF MICROBIOLOGY,2015,197(6):823—831.
APA Liu, HC,Xia, JL,Nie, ZY,Zhen, XJ,Zhang, LJ,&Xia, JL .(2015).Differential expression of extracellular thiol groups of moderately thermophilic Sulfobacillus thermosulfidooxidans and extremely thermophilic Acidianus manzaensis grown on S-0 and Fe2+.ARCHIVES OF MICROBIOLOGY,197(6),823—831.
MLA Liu, HC,et al."Differential expression of extracellular thiol groups of moderately thermophilic Sulfobacillus thermosulfidooxidans and extremely thermophilic Acidianus manzaensis grown on S-0 and Fe2+".ARCHIVES OF MICROBIOLOGY 197.6(2015):823—831.
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