CAS OpenIR  > 中科院上海应用物理研究所2011-2019年
Elaborately designed diblock nanoprobes for simultaneous multicolor detection of microRNAs
Wang, CG; Zhang, H; Zeng, DD; Sun, WL; Zhang, HL; Aldalbahi, A; Wang, YS; San, LL; Fan, CH; Zuo, XL; Mi, XQ; Zuo, XL (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, Div Phys Biol, Shanghai 201800, Peoples R China.
2015
Source PublicationNANOSCALE
ISSN2040-3364
Volume7Issue:38Pages:15822—15829
Subtype期刊文献
AbstractSimultaneous detection of multiple biomarkers has important prospects in the biomedical field. In this work, we demonstrated a novel strategy for the detection of multiple microRNAs (miRNAs) based on gold nanoparticles (Au NPs) and polyadenine (polyA) mediated nanoscale molecular beacon (MB) probes (denoted p-nanoMBs). Novel fluorescent labeled p-nanoMBs bearing consecutive adenines were designed, of which polyA served as an effective anchoring block binding to the surface of Au NPs, and the appended hairpin block formed an upright conformation that favored the hybridization with targets. Using the co-assembling method and the improved hybridization conformation of the hairpin probes, we achieved high selectivity for specifically distinguishing DNA targets from single-base mismatched DNA targets. We also realized multicolor detection of three different synthetic miRNAs in a wide dynamic range from 0.01 nM to 200 nM with a detection limit of 10 pM. What's more, we even detected miRNAs in a simulated serum environment, which indicated that our method could be used in complex media. Compared with the traditional method, our strategy provides a promising alternative method for the qualitative and quantitative detection of miRNAs.
KeywordResonance Energy-transfer Molecular Beacons Gold Nanoparticles Living Cells Nucleic-acid Dna Hybridization Oligonucleotide Amplification Probes
Indexed BySCI
Language英语
WOS IDWOS:000361834100034
Citation statistics
Cited Times:29[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/24913
Collection中科院上海应用物理研究所2011-2019年
Corresponding AuthorZuo, XL (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, Div Phys Biol, Shanghai 201800, Peoples R China.
Recommended Citation
GB/T 7714
Wang, CG,Zhang, H,Zeng, DD,et al. Elaborately designed diblock nanoprobes for simultaneous multicolor detection of microRNAs[J]. NANOSCALE,2015,7(38):15822—15829.
APA Wang, CG.,Zhang, H.,Zeng, DD.,Sun, WL.,Zhang, HL.,...&Zuo, XL .(2015).Elaborately designed diblock nanoprobes for simultaneous multicolor detection of microRNAs.NANOSCALE,7(38),15822—15829.
MLA Wang, CG,et al."Elaborately designed diblock nanoprobes for simultaneous multicolor detection of microRNAs".NANOSCALE 7.38(2015):15822—15829.
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