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| Elaborately designed diblock nanoprobes for simultaneous multicolor detection of microRNAs | |
| Wang, CG; Zhang, H; Zeng, DD; Sun, WL; Zhang, HL; Aldalbahi, A; Wang, YS; San, LL; Fan, CH; Zuo, XL; Mi, XQ; Zuo, XL (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, Div Phys Biol, Shanghai 201800, Peoples R China. | |
| 2015 | |
| Source Publication | NANOSCALE
 |
| ISSN | 2040-3364 |
| Volume | 7Issue:38Pages:15822—15829 |
| Subtype | 期刊文献 |
| Abstract | Simultaneous detection of multiple biomarkers has important prospects in the biomedical field. In this work, we demonstrated a novel strategy for the detection of multiple microRNAs (miRNAs) based on gold nanoparticles (Au NPs) and polyadenine (polyA) mediated nanoscale molecular beacon (MB) probes (denoted p-nanoMBs). Novel fluorescent labeled p-nanoMBs bearing consecutive adenines were designed, of which polyA served as an effective anchoring block binding to the surface of Au NPs, and the appended hairpin block formed an upright conformation that favored the hybridization with targets. Using the co-assembling method and the improved hybridization conformation of the hairpin probes, we achieved high selectivity for specifically distinguishing DNA targets from single-base mismatched DNA targets. We also realized multicolor detection of three different synthetic miRNAs in a wide dynamic range from 0.01 nM to 200 nM with a detection limit of 10 pM. What's more, we even detected miRNAs in a simulated serum environment, which indicated that our method could be used in complex media. Compared with the traditional method, our strategy provides a promising alternative method for the qualitative and quantitative detection of miRNAs. |
| Keyword | Resonance Energy-transfer Molecular Beacons Gold Nanoparticles Living Cells Nucleic-acid Dna Hybridization Oligonucleotide Amplification Probes |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000361834100034 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/24913 |
| Collection | 中科院上海应用物理研究所2011-2020年 |
| Corresponding Author | Zuo, XL (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, Div Phys Biol, Shanghai 201800, Peoples R China. |
| Recommended Citation GB/T 7714 | Wang, CG,Zhang, H,Zeng, DD,et al. Elaborately designed diblock nanoprobes for simultaneous multicolor detection of microRNAs[J]. NANOSCALE,2015,7(38):15822—15829. |
| APA | Wang, CG.,Zhang, H.,Zeng, DD.,Sun, WL.,Zhang, HL.,...&Zuo, XL .(2015).Elaborately designed diblock nanoprobes for simultaneous multicolor detection of microRNAs.NANOSCALE,7(38),15822—15829. |
| MLA | Wang, CG,et al."Elaborately designed diblock nanoprobes for simultaneous multicolor detection of microRNAs".NANOSCALE 7.38(2015):15822—15829. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| Elaborately designed（1598KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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