Nanoprobe-Initiated Enzymatic Polymerization for Highly Sensitive Electrochemical DNA Detection
Wan, Y; Wang, PJ; Su, Y; Wang, LH; Pan, D; Aldalbahi, A; Yang, SL; Zuo, XL; Yang, SL (reprint author), Nanjing Univ Sci & Technol, Sch Environm & Biol Engn, Nanjing 210094, Jiangsu, Peoples R China.
2015
发表期刊ACS APPLIED MATERIALS & INTERFACES
ISSN1944-8244
卷号7期号:46页码:25618—25623
文章类型期刊文献
摘要Electrochemical DNA (E-DNA) sensors have been greatly developed and play an important role in early diagnosis of different diseases. To determine the extremely low abundance of DNA biomarkers in clinical samples, scientists are making unremitting efforts toward achieving highly sensitive and selective E-DNA sensors. Here, a novel E-DNA sensor was developed taking advantage of the signal amplification efficiency of nanoprobe-initiated enzymatic polymerization (NIEP). In the NIEP based E-DNA sensor, the capture probe DNA was thiolated at its 3'-terminal to be immobilized onto gold electrode, and the nanoprobe was fabricated by 5'-thiol-terminated signal probe DNA conjugated gold nanoparticles (AuNPs). Both of the probes could simultaneously hybridize with the target DNA to form a sandwich structure followed by the terminal deoxynucleotidyl transferase (TdT)-catalyzed elongation of the free 3'-terminal of DNA on the nanoprobe. During the DNA elongation, biotin labels were incorporated into the NIEP-generated long single-stranded DNA (ssDNA) tentacles, leading to specific binding of avidin modified horseradish peroxidase (Av-HRP). Since there are hundreds of DNA probes on the nanoprobe, one hybridization event would generate hundreds of long ssDNA tentacles, resulting in tens of thousands of HRP catalyzed reduction of hydrogen peroxide and sharply increasing electrochemical signals. By employing nanoprobe and TdT, it is demonstrated that the NIEP amplified E-DNA sensor has a detection limit of 10 fM and excellent differentiation ability for even single-base mismatch.
关键词Rolling Circle Amplification Sequence-specific Detection Label-free Signal Amplification Strand-displacement Gold Nanoparticles Femtomolar Dna Chronocoulometric Dna Chain-reaction Nucleic-acids
语种英语
WOS记录号WOS:000366005600010
引用统计
文献类型期刊论文
条目标识符http://ir.sinap.ac.cn/handle/331007/24999
专题中科院上海应用物理研究所2011-2018年
通讯作者Yang, SL (reprint author), Nanjing Univ Sci & Technol, Sch Environm & Biol Engn, Nanjing 210094, Jiangsu, Peoples R China.
推荐引用方式
GB/T 7714
Wan, Y,Wang, PJ,Su, Y,et al. Nanoprobe-Initiated Enzymatic Polymerization for Highly Sensitive Electrochemical DNA Detection[J]. ACS APPLIED MATERIALS & INTERFACES,2015,7(46):25618—25623.
APA Wan, Y.,Wang, PJ.,Su, Y.,Wang, LH.,Pan, D.,...&Yang, SL .(2015).Nanoprobe-Initiated Enzymatic Polymerization for Highly Sensitive Electrochemical DNA Detection.ACS APPLIED MATERIALS & INTERFACES,7(46),25618—25623.
MLA Wan, Y,et al."Nanoprobe-Initiated Enzymatic Polymerization for Highly Sensitive Electrochemical DNA Detection".ACS APPLIED MATERIALS & INTERFACES 7.46(2015):25618—25623.
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