CAS OpenIR  > 中科院上海应用物理研究所2011-2020年
Novel DNA Polymer for Amplification Pretargeting
Li, X; Huang, QQ; Xiao, J; Liu, GZ; Dou, SP; Rusckowski, M; Shi, HC; Liu, YX; Cheng, DF; Liu, YX (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China.
AbstractIn this Letter, different from conventional pretargeting, an additional novel DNA polymer with multiple copies of a target was first designed to be administrated between the antitumor antibody, and the labeled effector served as an amplification pretargeting strategy. Two phosphorothioate DNA strands, a bridging and a target strand, were hybridized to form a polymer. Polymer size, as a function of molar ratios, was then monitored by size exclusion HPLC and electrophoretic mobility shift assay. Moreover, binding efficiency of polymers with the radiolabeled effector and polymer size after hybridization were measured by HPLC as well. As the polymer was expected to produce more binding sites that would be targeted by effectors, amplification pretargeting can greatly improve accumulation of effectors in tumor. This novel proof-of-concept was then well demonstrated by the in vitro test of signal amplification in antibody-binding protein L coated plate and LS174T cells. Compared to conventional pretargeting, significantly increasing radioactive signal was observed in this designed amplification pretargeting, which would serve as a useful paradigm of the potential of oligomer polymers to improve pretargeting and other related approaches.
KeywordAntibody Antigen Immunoscintigraphy Cancer Radioimmunotherapy Streptavidin Morpholino Strategy B72.3 Mice
WOS IDWOS:000361259800006
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Document Type期刊论文
Corresponding AuthorLiu, YX (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China.
Recommended Citation
GB/T 7714
Li, X,Huang, QQ,Xiao, J,et al. Novel DNA Polymer for Amplification Pretargeting[J]. ACS MEDICINAL CHEMISTRY LETTERS,2015,6(9):972—976.
APA Li, X.,Huang, QQ.,Xiao, J.,Liu, GZ.,Dou, SP.,...&Liu, YX .(2015).Novel DNA Polymer for Amplification Pretargeting.ACS MEDICINAL CHEMISTRY LETTERS,6(9),972—976.
MLA Li, X,et al."Novel DNA Polymer for Amplification Pretargeting".ACS MEDICINAL CHEMISTRY LETTERS 6.9(2015):972—976.
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