Poly-adenine-based programmable engineering of gold nanoparticles for highly regulated spherical DNAzymes
Zhu, D; Pei, H; Chao, J; Su, S; Aldalbahi, A; Rahaman, M; Wang, LH; Wang, LH; Huang, W; Fan, CH; Zuo, XL; Zuo, XL (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Div Phys Biol, Shanghai 201800, Peoples R China.
2015
发表期刊NANOSCALE
ISSN2040-3364
卷号7期号:44页码:18671—18676
文章类型期刊文献
摘要Enzyme complexes are assembled at the two-dimensional lipid membrane or prearranged on three-dimensional scaffolding proteins to regulate their catalytic activity in cells. Inspired by nature, we have developed gold nanoparticle-based spherical DNAzymes (SNAzymes) with programmably engineered activities by exploiting poly-adenine (polyA)-Au interactions. In a SNAzyme, AuNPs serve as the metal core, which is decorated with a functional shell of DNAzymes. Conventional thiolated DNAzyme-based assembly leads to disordered structures with suppressed activity. In contrast, by using an anchoring block of polyA tails, we find that the activity of SNAzymes can be programmably regulated. By using a polyA30 tail, SNAzymes demonstrated remarkably enhanced binding affinity compared to the thiolated DNAzyme-based assembly (similar to 75-fold) or individual DNAzymes in the solution phase (similar to 10-fold). More significantly, this increased affinity is directly translated to the sensitivity improvement in the SNAzyme-based lead sensor. Hence, this design of SNAzymes may provide new opportunities for developing biosensors and bioimaging probes for theranostic applications.
关键词Nucleic-acids Gene-regulation Living Cells Scaffold Proteins Nano-flares Uranyl-ion Delivery Oligonucleotides Efficiency Mechanism
语种英语
WOS记录号WOS:000364824000036
引用统计
被引频次:16[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.sinap.ac.cn/handle/331007/25016
专题中科院上海应用物理研究所2011-2018年
通讯作者Zuo, XL (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Div Phys Biol, Shanghai 201800, Peoples R China.
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GB/T 7714
Zhu, D,Pei, H,Chao, J,et al. Poly-adenine-based programmable engineering of gold nanoparticles for highly regulated spherical DNAzymes[J]. NANOSCALE,2015,7(44):18671—18676.
APA Zhu, D.,Pei, H.,Chao, J.,Su, S.,Aldalbahi, A.,...&Zuo, XL .(2015).Poly-adenine-based programmable engineering of gold nanoparticles for highly regulated spherical DNAzymes.NANOSCALE,7(44),18671—18676.
MLA Zhu, D,et al."Poly-adenine-based programmable engineering of gold nanoparticles for highly regulated spherical DNAzymes".NANOSCALE 7.44(2015):18671—18676.
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