CAS OpenIR  > 中科院上海应用物理研究所2011-2018年
Poly-adenine-based programmable engineering of gold nanoparticles for highly regulated spherical DNAzymes
Zhu, D; Pei, H; Chao, J; Su, S; Aldalbahi, A; Rahaman, M; Wang, LH; Wang, LH; Huang, W; Fan, CH; Zuo, XL; Zuo, XL (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Div Phys Biol, Shanghai 201800, Peoples R China.
2015
Source PublicationNANOSCALE
ISSN2040-3364
Volume7Issue:44Pages:18671—18676
Subtype期刊文献
AbstractEnzyme complexes are assembled at the two-dimensional lipid membrane or prearranged on three-dimensional scaffolding proteins to regulate their catalytic activity in cells. Inspired by nature, we have developed gold nanoparticle-based spherical DNAzymes (SNAzymes) with programmably engineered activities by exploiting poly-adenine (polyA)-Au interactions. In a SNAzyme, AuNPs serve as the metal core, which is decorated with a functional shell of DNAzymes. Conventional thiolated DNAzyme-based assembly leads to disordered structures with suppressed activity. In contrast, by using an anchoring block of polyA tails, we find that the activity of SNAzymes can be programmably regulated. By using a polyA30 tail, SNAzymes demonstrated remarkably enhanced binding affinity compared to the thiolated DNAzyme-based assembly (similar to 75-fold) or individual DNAzymes in the solution phase (similar to 10-fold). More significantly, this increased affinity is directly translated to the sensitivity improvement in the SNAzyme-based lead sensor. Hence, this design of SNAzymes may provide new opportunities for developing biosensors and bioimaging probes for theranostic applications.
KeywordNucleic-acids Gene-regulation Living Cells Scaffold Proteins Nano-flares Uranyl-ion Delivery Oligonucleotides Efficiency Mechanism
Language英语
WOS IDWOS:000364824000036
Citation statistics
Cited Times:17[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/25016
Collection中科院上海应用物理研究所2011-2018年
Corresponding AuthorZuo, XL (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Div Phys Biol, Shanghai 201800, Peoples R China.
Recommended Citation
GB/T 7714
Zhu, D,Pei, H,Chao, J,et al. Poly-adenine-based programmable engineering of gold nanoparticles for highly regulated spherical DNAzymes[J]. NANOSCALE,2015,7(44):18671—18676.
APA Zhu, D.,Pei, H.,Chao, J.,Su, S.,Aldalbahi, A.,...&Zuo, XL .(2015).Poly-adenine-based programmable engineering of gold nanoparticles for highly regulated spherical DNAzymes.NANOSCALE,7(44),18671—18676.
MLA Zhu, D,et al."Poly-adenine-based programmable engineering of gold nanoparticles for highly regulated spherical DNAzymes".NANOSCALE 7.44(2015):18671—18676.
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